Growth and instability of a phospholipid vesicle in a bath of fatty acids
Growth and instability of a phospholipid vesicle in a bath of fatty acids
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DOI：
10.1140/epjp/i2017-11554-1
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发表时间：
2017-06-29
影响因子：
3.4
通讯作者：
Libchaber, A. J.
中科院分区：
文献类型：
--
作者：
Dervaux, J.;Noireaux, V.;Libchaber, A. J.
使用微流控阱，我们研究了与脂肪酸接触的单个磷脂囊泡的行为。我们发现，自发的脂肪酸插入内的双层囊泡的大小，跨膜的渗透压差和脂肪酸浓度在外部浴控制。根据这些参数，囊泡可以球形生长或变得不稳定并分裂成几个子囊泡。我们建立了泡生长的相图，我们推导出一个简单的热力学模型，再现了泡体积的时间演化。最后，我们表明，稳定的增长，可以实现人工细胞表达一组简单的细菌细胞骨架蛋白，铺平了道路，人工细胞繁殖。
Using a microfluidic trap, we study the behavior of individual phospholipid vesicles in contact with fatty acids. We show that spontaneous fatty acids insertion inside the bilayer is controlled by the vesicle size, osmotic pressure difference across the membrane and fatty acids concentration in the external bath. Depending on these parameters, vesicles can grow spherically or become unstable and fragment into several daughter vesicles. We establish the phase diagram for vesicle growth and we derive a simple thermodynamic model that reproduces the time evolution of the vesicle volume. Finally, we show that stable growth can be achieved on an artificial cell expressing a simple set of bacterial cytoskeletal proteins, paving the way toward artificial cell reproduction.