Polyaniline/Prussian‐Blue‐Based Amperometric Biosensor for Detection of Uric Acid
Polyaniline/Prussian‐Blue‐Based Amperometric Biosensor for Detection of Uric Acid
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DOI：
10.1002/cplu.201200222
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发表时间：
2013-02
期刊：
影响因子：
3.4
通讯作者：
B. Thakur;S. Sawant
中科院分区：
文献类型：
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作者：
B. Thakur;S. Sawant
聚苯胺/普鲁士蓝（PANI-PB）复合材料以电化学方式沉积在铂电极上。通过使用循环伏安法，利用 PANI-PB 修饰电极对 H2O2 传感响应的特性，发现在低电位范围内比 PANI 修饰电极更有效。 PANI-PB 修饰电极通过固定尿酸酶进一步用于尿酸的生物分析。通过扫描电子显微镜 (SEM)、红外光谱和循环伏安法对电极在各个修饰阶段进行了表征。该生物传感器对尿酸浓度范围为 10–160 mm 呈线性响应，灵敏度为 160 m Am m × 1 cm × 2 。 PANI-PB 复合电极的主要优点是其用于测定尿酸的低工作电位（0 V vs. Ag/AgCl），这使得能够消除由实际样品中存在的分析物引起的可能的干扰效应。该生物传感器对尿酸具有选择性，对抗坏血酸、葡萄糖和尿素等分析物没有反应。如果在 188°C 下储存，传感器电极可在 7 天内保持其选择性和灵敏度。该生物传感器在检测人体血清样本中的尿酸方面也表现出令人满意的性能。
Polyaniline/Prussian blue (PANI-PB) composite was electrochemically deposited on a platinum electrode. The properties of the PANI-PB-modified electrode were exploited in terms of its response toward H2O2 sensing by using cyclic voltammetry, and were found to be more effectual than the PANI-modified electrode in the low-potential range. The PANI-PB-modified electrode was further used for the bioanalysis of uric acid by immobilization of the enzyme uricase. The electrode was characterized at various stages of modification by scanning electron microscopy (SEM), IR spectroscopy, and cyclic voltammetry. The biosensor gave a linear response for uric acid in the concentration range 10–160 mm with a sensitivity of 160 m Am m � 1 cm � 2 . The main advantage of the PANI-PB composite electrode is its low working potential (0 V vs. Ag/AgCl) for the estimation of uric acid, which enables the elimination of plausible interfering effects caused by analytes present in real samples. The biosensor was selective to uric acid and did not respond to analytes such as ascorbic acid, glucose, and urea. The sensor electrode retained its selectivity and sensitivity for seven days if stored at � 188C. The biosensor also displayed a satisfactory performance for the detection of uric acid in human blood serum samples.