Anti-Rhizoctonia solani activity by Desmos chinensis extracts and its mechanism of action
Anti-Rhizoctonia solani activity by Desmos chinensis extracts and its mechanism of action
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DOI：
10.1016/j.cropro.2012.09.004
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发表时间：
2013-01-01
期刊：
影响因子：
2.8
通讯作者：
Voravuthikunchai, Supayang Piyawan
中科院分区：
文献类型：
--
作者：
Plodpai, Patimaporn;Chuenchitt, Samerchai;Voravuthikunchai, Supayang Piyawan
本研究旨在探讨假鹰爪提取物防治水稻纹枯病的作用机制，并评价其应用潜力。D.结果表明，中国大豆对水稻立枯病菌具有较高的抑菌活性。提取物显示具有抗R。茄病活性，最小抑制浓度（MIC）和最小杀真菌浓度值分别为31.2至62.5 μ g mL（-1）和62.5至500 μ g mL（-1）。在薄层层析板上的生物自显影显示提取物的抗真菌活性，R-f值为0.33。采用气相色谱-质谱联用技术，共检测鉴定出7种化合物，分别为苯甲酸苄酯和2-羟基苯甲酸苄酯、2种倍半萜类化合物（α-桉叶醇和β-桉叶醇）、1种芳香醇（苯甲醇）、1种芳香酮（苯乙酮）和1种二萜类化合物（植醇）。电子显微照片证实了提取物对处理过的真菌细胞的形态和超微结构改变的影响。用4 MIC的提取物处理的菌丝体的显微照片示出了异常的形态学，例如沿着菌丝体表面的收缩、部分扭曲和不同大小的球形结构。破坏膜结构，包括细胞膜的破坏，核膜的部分损失，以及菌丝细胞质和膜细胞器的耗尽。叶面喷施D. 2 mg·mL ~（-1）的中华绒螯蟹提取物对水稻纹枯病有显著的抑制作用。结果表明，D.中国提取物可用作防治水稻纹枯病的植物性杀真菌剂。皇冠版权所有（C）2012由爱思唯尔有限公司出版。保留所有权利。
The objectives of this study were to investigate the mechanisms of action and to evaluate the potential application of Desmos chinensis extracts for controlling rice sheath blight. The dichloromethane extract from D. chinensis demonstrated high antifungal activity against Rhizoctonia solani. The extract was shown to have anti-R. solani activity with minimum inhibitory concentration (MIC) and minimum fungicidal concentration values ranging from 31.2 to 62.5 mu g mL(-1) and from 62.5 to 500 mu g mL(-1), respectively. Bioautography on thin-layer chromatography plates demonstrated antifungal activity of the extract with an R-f value of 0.33. A total of 7 compounds, 2 benzoate esters (benzyl benzoate and benzyl 2-hydroxybenzoate), 2 sesquiterpenoids (alpha-eudesmol and beta-eudesmol), 1 aromatic alcohol (benzyl alcohol), I aromatic ketone (acetophenone) and 1 diterpenoid (phytol), were detected and identified using gas chromatography-mass spectrometry analysis. Electron micrographs confirmed the effects of the extract on morphological and ultrastructural alterations in the treated fungal cells. The micrographs of the mycelia treated with the extract at 4MIC illustrated aberrant morphology such as shrinkage, partial distortion and globular structures of different sizes along the surface of the mycelia. Damaging membranous structures including disruption of the cell membrane, partial loss of nuclear membranes, and depletion of hyphal cytoplasm and membranous organelles were observed. Foliar application of D. chinensis extract at a concentration of 2 mg mL(-1) on rice markedly decreased sheath blight severity. It was concluded that the application of D. chinensis extract can be used as a botanical fungicide to control rice sheath blight. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.