Growth at low temperature suppresses readthrough of the UGA stop codon during the expression of Bacillus subtilis flgM gene in Escherichia coli
Growth at low temperature suppresses readthrough of the UGA stop codon during the expression of Bacillus subtilis flgM gene in Escherichia coli
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DOI：
10.1016/s0168-1656(02)00340-1
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发表时间：
2003-03-06
影响因子：
4.1
通讯作者：
Galizzi, A
中科院分区：
文献类型：
--
作者：
González, B;Ceciliani, F;Galizzi, A
在大肠杆菌中高效生产重组蛋白需要适当终止翻译以确保仅合成所需产物。在大肠杆菌中重组生产枯草芽孢杆菌flgM的过程中，我们检测到分子量高于预期的额外多肽，对应于UGA终止密码子的翻译通读产物。在本文中，我们表明，通读被废除时，重组蛋白的合成进行在25 degreesC。可能的原因，有助于减少对正确的终止产品的通读蛋白质物种的比例进行了讨论。(C)2002 Elsevier Science B.V.保留所有权利。
The efficient production of recombinant proteins in Escherichia coli requires a proper termination of translation to ensure the synthesis of only the desired product. During the recombinant production of Bacillus subtilis flgM in E coli, we detected an additional polypeptide of molecular mass higher than the expected, corresponding to a product of a translational readthrough of the UGA stop codon. In this paper we show that the readthrough was abolished when the synthesis of the recombinant protein was carried out at 25 degreesC. The possible causes that contribute to reduce the proportion of readthrough protein species against the correct terminated product are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.