Phosphopeptide enrichment by IEF
Phosphopeptide enrichment by IEF
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DOI：
10.1002/elps.200600145
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发表时间：
2006-11-01
期刊：
影响因子：
2.9
通讯作者：
Turck, Christoph W.
中科院分区：
文献类型：
--
作者：
Maccarrone, Giuseppina;Kolb, Nikolaus;Turck, Christoph W.
为了改进 MS 对磷酸肽的鉴定，我们在 IPG 试纸条上使用了肽 IEF。源自胰蛋白酶消化的单一蛋白质以及复杂的细胞蛋白质混合物的磷酸肽可以通过 IEF 进行富集，并在 IPG 试纸条的酸性末端以优异的产量进行回收。 IPG 肽分级分离与 MS/MS 分析相结合，使我们能够从细胞蛋白提取物的胰蛋白酶消化物中鉴定磷酸肽。
In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract.