Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG].
Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG].
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特异烷基化的 DNA 片段。
DOI：
10.1093/nar/11.10.3393
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发表时间：
1983
影响因子：
14.9
通讯作者：
Jones,RA
中科院分区：
文献类型：
--
作者：
Kuzmich,S;Marky,LA;Jones,RA
用固相磷酸三酯法合成了两个含有致癌修饰碱基06-甲基鸟嘌呤的六聚体DNA片段，其中未修饰的鸟嘌呤残基被4-硝基苯乙基保护。这两个烷基化寡核苷酸的Tm相似，比未修饰的母体化合物d(CG)3低约40。此外，CD光谱分析表明，(06 Me)G的存在似乎抑制了B-Z转变
Two hexamer DNA fragments containing a carcinogenic modified base, 06-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were 0oprotected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40olower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (06Me)G appears to inhibit the B+Z transition, as determined by CD spectroscopy