Effect of acute and regular exercise on growth hormone secretagogue receptor-1a expression in human lymphocytes, T cell subpopulation and monocytes
Effect of acute and regular exercise on growth hormone secretagogue receptor-1a expression in human lymphocytes, T cell subpopulation and monocytes
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DOI：
10.1016/j.bbi.2013.09.017
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发表时间：
2014-07-01
影响因子：
15.1
通讯作者：
Stensel, David J.
中科院分区：
文献类型：
--
作者：
Bishop, Nicolette C.;Hayashida, Harumi;Stensel, David J.
促食欲肽激素ghrelin通过T细胞和单核细胞上的生长激素促分泌素受体-1a（GHS-R1 a）对促炎细胞因子释放发挥有效的抑制作用。因此，生长激素释放肽是一种有前途的治疗剂，用于治疗炎症性疾病，但这些效果取决于GHS-R1 a的可用性。本研究的目的是确定急性运动对循环CD 14+单核细胞、总淋巴细胞和CD 3 + T细胞上GHS-R1 a表达的影响。在一项随机交叉设计中，9名男性俱乐部标准自行车运动员以75%（V）超过dotO（2）峰值（EX）或休息（REST）骑自行车1小时。与相同时间的REST相比，EX中的循环GHS-R1 a+淋巴细胞和单核细胞的浓度在运动后立即以及运动后1和2 h均较高（均p <0.05）。仅在运动后即刻，EX中的CD 3 + GHS-R1 a+细胞浓度高于REST（258（203）个细胞μ l（-1）vs. 62（42）个细胞μ l（-1），p <0.05）。GHS-R1 a受体表达密度不受试验或时间的影响。将静止时的活跃参与者与7名年龄、性别和BMI匹配的久坐对照者进行比较，发现活跃男性的GHS-R1 a+淋巴细胞浓度较高（p <0.05）。这些研究结果表明，急性和定期运动的优先招聘的特定细胞亚群表达GHS-R1 a进入外周循环。鉴于ghrelin的抗炎作用取决于GHS-R1 a的可用性，这里发现的具有高抗炎潜力的亚群的优先招募为运动降低促炎细胞因子水平的潜在机制增加了一个新的方面。皇冠版权所有（C）2013由Elsevier Inc.发布。All rights reserved.
The orexigenic peptide hormone ghrelin exerts potent inhibitory effects on pro-inflammatory cytokine release via the growth hormone secretagogue receptor-1a (GHS-R1a) on T cells and monocytes. As such, ghrelin is a promising therapeutic agent for the treatment of inflammatory conditions, but these effects depend on the availability of GHS-R1a. The aim of this study was to determine the effect of acute exercise on GHS-R1a expression on circulating CD14+ monocytes, total lymphocytes and CD3+ T cells. Nine male club-standard cyclists cycled for 1 h at 75% (V)over dotO(2)peak (EX) or rested (REST) in a randomised cross-over design. Compared with the equivalent times in REST, the concentration of circulating GHS-R1a+ lymphocytes and monocytes was higher in EX at immediately and 1 and 2 h post-exercise (all p < .05). The concentration of CD3+GHS-R1a+ cells was higher in EX than in REST immediately post-exercise only (258 (203) cells mu l(-1) vs. 62 (42) cells mu l(-1), p < .05). Density of GHS-R1a receptor expression was unaffected by trial or time. Comparison of active participants at rest with 7 age-, sex- and BMI-matched sedentary controls revealed a higher concentration of GHS-R1a+ lymphocytes in active males (p < .05). These findings suggest a preferential recruitment of specific cell subpopulations expressing GHS-R1a into the peripheral circulation with acute and regular exercise. Given that the anti-inflammatory effects of ghrelin depend on the availability of GHS-R1a, the preferential recruitment of subpopulations with high anti-inflammatory potential found here add a novel aspect to the potential mechanisms by which exercise acts to reduce pro-inflammatory cytokine levels. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.