3 ASSAYS FOR THE CHARACTERIZATION AND QUANTITATION OF HUMAN-SERUM AMYLOID-A
3 ASSAYS FOR THE CHARACTERIZATION AND QUANTITATION OF HUMAN-SERUM AMYLOID-A
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DOI：
10.1111/j.1365-3083.1983.tb01804.x
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
MARHAUG, G
中科院分区：
文献类型：
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作者：
MARHAUG, G
开发了两种不同的放射免疫测定法 (RIA) 和酶联免疫吸附测定法 (ELISA)，用于 [人] 淀粉样蛋白 A (AA) 和血清淀粉样蛋白 A (SAA) 蛋白的定量和抗原表征，并对这 3 种测定法进行了评估和比较。考虑了灵敏度、重现性、血清变性和储存的影响以及测定范围。所有 3 种检测都有用，但目的不同。最适合测定全血清中 SAA 的方法是第二抗体沉淀 RIA，以纯化的 SAA 作为标记示踪剂和标准品，并以多克隆兔抗 SAA 作为第一抗体。该测定提供了绝对量 (mg/l) 的 SAA 浓度和可接受的重现性，无需事先对血清进行变性。观察了使用 SAA 单克隆抗体的 ELISA 和使用 AA、SAA、抗 AA 和抗 SAA 的固相 RIA 的优点和缺点。这 3 种检测方法适用于 AA 和 SAA 的抗原研究。
Two different radioimmunoassays (RIA) and an enzyme-linked immunosorbent assay (ELISA) were developed for the quantitation and antigenic characterization of [human] amyloid A (AA) and serum amyloid A (SAA) proteins, and the 3 assays were evaluated and compared with each other. Sensitivity, reproducibility, effect of denaturation and storage of serum and range of determination were considered. All 3 assays were useful, but for different purposes. The most suitable method for the determination of SAA in whole serum was a 2nd antibody precipitation RIA with purified SAA as labeled tracer and standard, and polyclonal rabbit anti-SAA as 1st antibody. This assay provided SAA concentrations in absolute amounts (mg/l) and acceptable reproducibility without need for prior denaturation of serum. Both advantages and disadvantages of ELISA using monoclonal antibodies to SAA and a solid-phase RIA using AA, SAA, anti-AA and anti-SAA were observed. The 3 assays were suitable for antigenic studies of AA and SAA.