Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4.
Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4.
复制标题
编码两种人 α 类谷胱甘肽转移酶（GSTA3 和 GSTA4）的 cDNA 的鉴定以及 GSTA4-4 的异源表达。
作者：
P. Board
已在表达序列标签数据库中检索了先前未描述的人α类谷胱甘肽转移酶的实例。在来自8-9周胎盘的cDNA文库中鉴定了先前描述的GSTA 3基因的不完全转录物。这表明GSTA 3基因是功能性的，并且可能在特定的发育调控下。在脑cDNA文库中鉴定了第二个cDNA，称为GSTA 4。编码的GSTA 4 -4酶在大肠杆菌中表达，发现其在免疫学上不同于GSTA 1 -1，并且对alk-2-enals具有高活性。尽管GSTA 4 -4似乎在功能上与小鼠GST 5.7和大鼠GST 8 -8 Alpha类酶相似，但序列比较和系统发育分析表明GSTA 4 -4可能是不同Alpha类亚组的成员。
The Expressed Sequence Tag database has been searched for examples of previously undescribed human Alpha class glutathione transferases. An incomplete transcript of the previously described GSTA3 gene was identified in a cDNA library derived from 8-9 week placenta. This indicates that the GSTA3 gene is functional and is possibly under specific developmental regulation. A second cDNA, termed GSTA4, was identified in a brain cDNA library. The encoded GSTA4-4 enzyme was expressed in Escherichia coli and was found to be immunologically distinct from GSTA1-1 and to have high activity with alk-2-enals. Although GSTA4-4 appears to be functionally similar to the mouse GST5.7 and rat GST8-8 Alpha class enzymes, sequence comparisons and phylogenetic analysis suggest that GSTA4-4 may be a member of a distinct Alpha class subgroup.