Effect of composition of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) on growth of fibroblast and osteoblast
Effect of composition of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) on growth of fibroblast and osteoblast
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DOI：
10.1016/j.biomaterials.2004.03.023
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发表时间：
2005-03-01
期刊：
影响因子：
14
通讯作者：
Chen, GQ
中科院分区：
文献类型：
--
作者：
Wang, YW;Yang, F;Chen, GQ
以聚（3-羟基丁酸酯）（PHB）、聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）和聚（L-丙交酯）（PLA）为对照，研究了由5%、12%和20%羟基己酸酯（HHx）组成的PHB、PHBHHx膜的生物医学应用。随着PHBHHx中HHx含量的增加，聚合物的表面性质发生相应的变化。P（HB-co-20%-HHx）具有最光滑的表面，而在所评价的PHB和所有PHBHHx中，PHB表面最亲水。所有PHBHHx也显示出较强的蛋白亲和性和生物相容性。结果发现，成纤维细胞和成骨细胞对这些聚合物有不同的反应：成纤维细胞喜欢P（HB-co-20%-HHx），而成骨细胞喜欢P（HB-co-12%-HHx）。与PLA相比，PHB和所有PHBHHx似乎对成纤维细胞和成骨细胞具有更好的生物相容性。具有不同表面性质的聚合物可以帮助满足不同的细胞要求。PHBHHx结合其良好的拉伸力学性能和可调的生物相容性，可满足不同组织和细胞生长的需要。(C)2004 Elsevier Ltd.保留所有权利。
Films made of poly (3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) consisting of 5% 12% and 20% hydroxyhexanoate (HHx), respectively, were evaluated for biomedical application in comparison with poly (L-Lactide) (PLA). With the increase of HHx content in PHBHHx, the polymer surface properties changed accordingly. P(HB-co-20%-HHx) had the smoothest Surface while PHB surface was most hydrophilic among the evaluated PHB and all the PHBHHx. All PHBHHx also showed strong protein affinity and biocompatibility. It was found that fibroblast and osteoblast had different responses to these polymers: fibroblast cells favored P(HB-co-20%-HHx), yet osteoblast cells preferred P(HB-co-12%-HHx). PHB and all PHBHHx appeared to have better biocompatibility for fibroblast and osteoblast compared with PLA. Polymers possessing diferent surface properties may help meet different cellular requirements. Combined with their good mechanical properties for elongation and adjustable biocompatibility, PHBHHx may meet the needs of growth requirements of different tissues and cells. (C) 2004 Elsevier Ltd. All rights reserved.