Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes
Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes
复制标题
DOI：
10.1038/ni928
复制
发表时间：
2003-06-01
影响因子：
30.5
通讯作者：
Robey, E
中科院分区：
文献类型：
--
作者：
Bousso, P;Robey, E
淋巴结中树突状细胞（DC）激活CD8（+）T细胞的细胞动力学尚不清楚。在这里，我们通过对完整的淋巴结进行实时双光子显微镜检查来跟踪T细胞和DC的行为。我们发现，在没有抗原的情况下，DC每小时扫描至少500个不同的T细胞。携带抗原的DC在募集肽特异性T细胞方面非常有效，并且可以同时接合十个以上的T细胞。这些相互作用的持续时间是几个小时，而不是几分钟。相互作用的整体亲合力影响T细胞被DC稳定捕获的概率，为T细胞竞争提供了可能的基础。总之，我们的结果确定了促进淋巴结中有效的CD8（+）T细胞应答的细胞行为。
The cellular dynamics underlying activation of CD8(+) T cells by dendritic cells (DCs) in the lymph node are not known. Here we have tracked the behavior of T cells and DCs by subjecting intact lymph nodes to real-time two-photon microscopy. We show that DCs scan at least 500 different T cells per hour in the absence of antigen. Antigen-bearing DCs are highly efficient in recruiting peptide-specific T cells and can engage more than ten T cells simultaneously. The duration of these interactions is of the order of hours, not minutes. The overall avidity of the interaction influences the probability that T cells will be stably captured by DCs, providing a possible basis for T cell competition. Taken together, our results identify the cellular behaviors that promote an efficient CD8(+) T cell response in the lymph node.