Immunohistochemical localization of cannabinoid type 1 and vanilloid transient receptor potential vanilloid type 1 receptors in the mouse brain
Immunohistochemical localization of cannabinoid type 1 and vanilloid transient receptor potential vanilloid type 1 receptors in the mouse brain
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DOI：
10.1016/j.neuroscience.2006.02.074
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Di Marzo, V.
中科院分区：
文献类型：
--
作者：
Cristino, L.;De Petrocellis, L.;Di Marzo, V.
大麻素I型受体和瞬时受体电位香草素1型通道已被提出分别作为两类内源性多不饱和脂肪酸酰胺，酰基乙醇酰胺和酰基多巴胺的代谢型和离子型受体。此外，我们和其他人已经表明，当这两种受体在同一细胞中表达时，它们之间会发生功能性串扰。虽然证明在背根神经节，脊髓和肌间神经元的感觉神经元，大麻素I型和瞬时受体电位香草素1型的共表达尚未在大脑中进行研究。在本研究中，我们通过使用市售的特异性抗体来解决这个问题，所述抗体的特异性通过从大麻素1型（-/-）和瞬时受体电位香草素1型（-/-）小鼠的大脑获得的数据来证实。双大麻素1型/瞬时受体电位香草素I型免疫荧光和单大麻素I型或瞬时受体电位香草素I型抗生物素蛋白-生物素复合物免疫组织化学技术进行，两种方法使用点相同的结果。在海马、基底节、丘脑、下丘脑、大脑脚、脑桥核、导水管周围灰质、小脑皮质和小脑齿状核中观察到大麻素1型/瞬时受体电位香草精1型表达。特别是，在海马中，在整个CA 1-CA 3亚区和齿状回分子层的许多锥体神经元的细胞体上检测到大麻素1型/瞬时受体电位香草素1型表达。在小脑皮质，大麻素1型/瞬时受体电位香草素I型受体的表达被发现周围的索马和轴突的绝大多数浦肯野细胞体，其细胞质被发现未染色的两种受体。大麻素I型和瞬时受体电位香草酸I型免疫反应阳性细胞还见于：a）苍白球和黑质，在致密和细小的大麻素I型/瞬时受体电位香草酸I型阳性和大麻素I型阳性神经纤维网中分别发现一些强烈的瞬时受体电位香草酸I型免疫反应阳性细胞体; B）丘脑和下丘脑神经元的细胞质;和c）腹侧导水管周围灰质的一些神经元。这些数据支持CNS中两种受体类型之间的功能关系的假设。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Cannabinold type I receptors and transient receptor potential vanilloid type 1 channels have been proposed to act as metabotropic and ionotropic receptors, respectively, for two classes of endogenous polyunsaturated fatty acid amides, the acylethanolamides and the acyldopamides. Furthermore, we and others have shown that functional crosstalk occurs between these two receptors when they are expressed in the same cell. Although demonstrated in sensory neurons of the dorsal root ganglia, spinal cord and myenteric neurons, co-expression of cannabinoid type I and transient receptor potential vanilloid type 1 has not yet been studied in the brain. In the present study, we addressed this issue by using commercially available specific antibodies whose specificity was confirmed by data obtained with brains from cannabinoid type 1(-/-) and transient receptor potential vanilloid type 1(-/-) mice. Double cannabinoid type 1/transient receptor potential vanilloid type I immunofluorescence and single cannabinoid type I or transient receptor potential vanilloid type I avidin-biotin complex immunohistochemistry techniques were performed and both methods used point to the same results. Cannabinoid type 1/transient receptor potential vanillold type 1 expression was observed in the hippocampus, basal ganglia, thalamus, hypothalamus, cerebral peduncle, Pontine nuclei, periaqueductal gray matter, cerebellar cortex and dentate cerebellar nucleus. In particular, in the hippocampus, cannabinoid type 1/transient receptor potential vanilloid type 1 expression was detected on cell bodies of many pyramidal neurons throughout the CA1-CA3 subfields and in the molecular layer of dentate gyrus. In the cerebellar cortex, expression of cannabinoid type 1/transient receptor potential vanilloid type I receptors was found surrounding soma and axons of the vast majority of Purkinje cell bodies, whose cytoplasm was found unstained for both receptors. Cannabinoid type I and transient receptor potential vanilloid type 1 immunoreactivity was also detected in: a) the globus pallidus and substantia nigra, in which some intensely transient receptor potential vanilloid type 1 immunopositive cell bodies were found in dense and fine cannabinoid type 1/transient receptor potential vanilloid type I positive and cannabinoid type I positive nerve fiber mesh-works, respectively; b) the cytoplasm of thalamic and hypothalamic neurons; and c) some neurons of the ventral periaqueductal gray. These data support the hypothesis of a functional relationship between the two receptor types in the CNS. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.