A novel rhabdovirus associated with acute hemorrhagic fever in central Africa.
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa.
复制标题
DOI：
10.1371/journal.ppat.1002924
复制
发表时间：
2012-09
期刊：
影响因子：
6.7
通讯作者：
Leroy EM
中科院分区：
文献类型：
--
作者：
Grard G;Fair JN;Lee D;Slikas E;Steffen I;Muyembe JJ;Sittler T;Veeraraghavan N;Ruby JG;Wang C;Makuwa M;Mulembakani P;Tesh RB;Mazet J;Rimoin AW;Taylor T;Schneider BS;Simmons G;Delwart E;Wolfe ND;Chiu CY;Leroy EM
利用深度测序发现了一种新型弹状病毒（巴斯刚果病毒，简称 BASV），该病毒与 2009 年非洲刚果民主共和国 (DRC) 曼加拉村爆发的 3 例人类急性出血热病例有关。这些病例的发病时间超过 3 周，其特点是突然发病、高烧、粘膜出血，其中两名患者在 3 天内死亡。在孤独幸存者的急性血清样本中检测到 BASV，浓度为 1.09×106 RNA 拷贝/mL，随后从约 1.4 亿个序列读取中重新组装了 98.2% 的基因组。系统发育分析表明，BASV 具有高度分化性，与任何其他弹状病毒的氨基酸同一性不到 34%。在幸存者和直接照顾他的无症状护士（两人都是医护人员）中检测到>1∶1000的高恢复期中和抗体滴度，表明BASV存在人际传播的可能性。该病毒的天然动物储存宿主或节肢动物载体以及精确的传播方式仍不清楚。 BASV 是一种与非洲急性出血热相关的新出现的人类病原体。我们使用深度测序（一种从临床样本中生成数百万条 DNA 序列读数的方法）来发现一种新型弹状病毒（巴斯刚果病毒，简称 BASV），该病毒与 2009 年非洲刚果民主共和国 (DRC) 曼加拉村爆发的 3 例人类急性出血热病例有关。这些病例的发病时间超过 3 周，特点是突然发病、高烧、血性呕吐和腹泻，其中两名患者在 3 天内死亡。 BASV 在唯一幸存者的血液中的浓度超过每毫升 100 万份。 BASV 的基因组由超过 1.4 亿条序列读取组装而成，表明它与任何其他弹状病毒都非常不同。唯一的幸存者和照顾他的一名护士（没有症状）都是医护人员，被发现具有高水平的 BASV 抗体，表明他们都已感染该病毒。尽管病毒来源尚不清楚，但我们的研究结果表明，BASV 可能通过人际接触传播，是一种与非洲急性出血热相关的新病原体。
Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09×106 RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ∼140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1∶1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa. We used deep sequencing, a method for generating millions of DNA sequence reads from clinical samples, to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, bloody vomiting and diarrhea, and, in two patients, death within 3 days. BASV was present in the blood of the lone survivor at a concentration of over a million copies per milliliter. The genome of BASV, assembled from over 140 million sequence reads, reveals that it is very different from any other rhabdovirus. The lone survivor and a nurse caring for him (with no symptoms), both health care workers, were found to have high levels of antibodies to BASV, indicating that they both had been infected by the virus. Although the source of the virus remains unclear, our study findings suggest that BASV may be spread by human-to-human contact and is an emerging pathogen associated with acute hemorrhagic fever in Africa.
登录
查看更多内容
影响因子：
3.6
作者：
Daniels, NA;Mackinnon, L;Mead, PS
通讯作者：
Mead, PS
影响因子：
3.8
作者：
Fichet-Calvet, Elisabeth;Rogers, David John
通讯作者：
Rogers, David John
影响因子：
3.7
作者：
Greninger AL;Chen EC;Sittler T;Scheinerman A;Roubinian N;Yu G;Kim E;Pillai DR;Guyard C;Mazzulli T;Isa P;Arias CF;Hackett J;Schochetman G;Miller S;Tang P;Chiu CY
通讯作者：
Chiu CY
影响因子：
6.4
作者：
Guimard, Y;Bwaka, MA;Kipasa, MA
通讯作者：
Kipasa, MA
影响因子：
14.9
作者：
Katoh K;Kuma K;Toh H;Miyata T
通讯作者：
Miyata T