EXUDATE POLYMORPHONUCLEAR LEUKOCYTES ISOLATED FROM SKIN CHAMBERS ARE PRIMED FOR ENHANCED RESPONSE TO SUBSEQUENT STIMULATION WITH CHEMOATTRACTANT F-MET-LEU-PHE AND C3-OPSONIZED YEAST PARTICLES
EXUDATE POLYMORPHONUCLEAR LEUKOCYTES ISOLATED FROM SKIN CHAMBERS ARE PRIMED FOR ENHANCED RESPONSE TO SUBSEQUENT STIMULATION WITH CHEMOATTRACTANT F-MET-LEU-PHE AND C3-OPSONIZED YEAST PARTICLES
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DOI：
10.1007/bf00916397
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发表时间：
1988-04-01
期刊：
影响因子：
5.1
通讯作者：
DAHLGREN, C
中科院分区：
文献类型：
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作者：
BRIHEIM, G;COBLE, B;DAHLGREN, C
研究了从无菌性炎症反应中分离的人多形核白细胞（PMNLs）对可溶性和颗粒物质刺激的代谢反应能力。平行研究了从同一个体的皮肤室分离的渗出物PMNL（E-PMNL）和从外周血分离的血液PMNL（B-PMNL）。与B-PMNL相比，E-PMNL被引发，导致对随后用趋化肽甲酰基-甲硫氨酰基-亮氨酰基-苯丙氨酸（FMLP）（334%）和血清调理的酵母颗粒（C3酵母）（201%）刺激的化学发光（CL）反应增加。另一方面，佛波醇肉豆蔻酸酯乙酸酯（PMA）诱导的CL反应在E-PMNLs中，只有70%的响应在B-PMNLs中获得。通过用细菌培养物滤液预处理，可以在B-PMNLs中诱导导致对FMLP和C3酵母的CL响应增强的类似引发状态。然而，用细菌培养物滤液预处理E-PMNL不会进一步增加对FMLP的CL反应。与B-PMNL相比，E-PMNL中对FMLP的增强的功能应答伴随着肽结合的增加，通过结合的f-Met-Leu-[3 H]Phe的量加倍（209%）证明。C3酵母诱导的CL产生E-PMNLs的增加伴随着C3调理酵母颗粒的摄入和附着的增加。然而，E-PMNLs中吞噬作用的增强依赖于所使用的调理素，因为IgG调理的酵母颗粒被E-PMNLs和B-PMNLs以相同的程度吞噬，从而表明选择性受体调节也参与E-PMNLs的引发以增强对C3酵母的响应。这些结果表明，从皮肤腔室分离的渗出液细胞相对于受体介导的功能进行调节，导致对FMLP的代谢反应增加，同时肽的结合增加和C3包被的酵母颗粒的吞噬作用增加。渗出过程中的受体调节可能是PMNLs调节炎症反应的重要机制。
The ability to respond metabolically to stimulation with both soluble and particulate substances was investigated in human polymorphonuclear leukocytes (PMNLs) isolated from an aseptic inflammatory reaction. Exudate PMNLs isolated from skin chambers (E-PMNLs) and blood PMNLs isolated from the peripheral blood (B-PMNLs) of the same individual were investigated in parallel. E-PMNLs were primed, resulting in an increased chemiluminescence (CL) response to subsequent stimulation with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) (334%) and serum-opsonized yeast particles (C3 yeast) (201%), as compared to B-PMNLs. Phorbol myristate acetate (PMA) on the other hand, induced a CL response in E-PMNLs that was only 70% of the response obtained in B-PMNLs. A similar primed state resulting in enhancement of the CL response to FMLP and C3 yeast could be induced in B-PMNLs by pretreatment with a bacterial culture filtrate. Pretreatment of E-PMNLs with the bacterial culture filtrate, however, did not increase the CL response to FMLPs any further. The enhanced functional response to FMLPs in E-PMNLs was accompanied by an increased binding of the peptide, demonstrated by a doubling of the amount of bound f-Met-Leu-[3H]Phe(209%), as compared to B-PMNLs. The increased C3-yeast-induced CL generation in E-PMNLs was accompanied by an increased ingestion and attachment of C3-opsonized yeast particles. The enhancement of phagocytosis in E-PMNLs was, however, dependent upon the opsonin used, since IgG-opsonized yeast particles were phagocytosed to the same extent by E-PMNLs and B-PMNLs, thereby indicating that selective receptor modulation is also involved in the priming of E-PMNLs for an enhanced response to C3-yeast. These results show that exudate cells isolated from skin chambers are modulated with respect to receptor-mediated functions resulting in an increased metabolic response to FMLP coupled with an increased binding of the peptide and an increased phagocytosis of C3-coated yeast particles. Receptor modulation during exudation may be an important mechanism in regulating the infammatory response by PMNLs.