Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm
Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm
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DOI：
10.1016/j.ydbio.2008.05.542
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发表时间：
2008-08-01
影响因子：
2.7
通讯作者：
Scotting, Paul J.
中科院分区：
文献类型：
--
作者：
Dee, Chris T.;Hirst, Caroline S.;Scotting, Paul J.
对于细胞外信号（如bmp和Fgfs）调节神经命运的第一个转录事件知之甚少。Sox3是发育中的中枢神经系统中最早表达的转录因子之一，并已被证明受这些信号通路的调节。我们使用斑马鱼的功能获得和功能丧失实验来阐明Sox3在决定神经命运中的作用。异位Sox3在外胚层细胞分化的早期诱导神经组织，并且这种作用得以维持，以至于大范围的额外中枢神经系统很明显，包括中枢神经系统的几乎完全复制。使用morpholinos敲除Sox3导致中枢神经系统、耳朵和眼睛的大小减小，并随后抑制神经发生的某些方面。我们的数据还表明，Sox3的亲神经作用可以补偿Fgf信号传导在诱导神经组织中的抑制作用，但不足以维持神经命运，这表明Fgf在后期阶段存在与Sox3无关的作用。(c) 2008爱思唯尔公司版权所有。
Little is known of the first transcriptional events that regulate neural fate in response to extracellular signals Such as Bmps and Fgfs. Sox3 is one of the earliest transcription factors to be expressed in the developing CNS and has been shown to be regulated by these signalling pathways. We have used both gain- and loss-of-function experiments in zebrafish to elucidate the role of Sox3 in determining neural fate. Ectopic Sox3 caused induction of neural tissue from a very early stage of cell specification in the ectoderm and this effect was maintained Such that large domains of additional CNS were apparent, including almost complete duplications of the CNS. Knock-clown of Sox3 using morpholinos resulted in a reduction in the size of the CNS, ears and eyes and subsequent inhibition of some aspects Of neurogenesis. Our data also Suggest that the pro-neural effects of Sox3 can compensate for inhibition of Fgf signalling in inducing neural tissue but it is not sufficient to maintain neural fate, suggesting the presence of Sox3-independent roles of Fgf at later stages. (c) 2008 Elsevier Inc. All rights reserved.