Creatine phosphate inhibition of adenylate deaminase is mainly due to pyrophosphate.
Creatine phosphate inhibition of adenylate deaminase is mainly due to pyrophosphate.
复制标题
磷酸肌酸对腺苷酸脱氨酶的抑制作用主要是由于焦磷酸盐。
DOI：
10.1016/s0021-9258(17)37794-3
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
J. Lowenstein
中科院分区：
文献类型：
--
作者：
T. J. Wheeler;J. Lowenstein
先前报道的肌酸磷酸对大鼠骨骼肌腺苷脱氨酶的抑制是由于无机焦磷酸盐作为一种污染物存在于肌酸磷酸的商业制剂中。这一结论是基于以下证据得出的：用离子交换色谱法可以从商业制备的磷酸肌酸中分离出抑制腺苷酸脱氨酶的化合物；用无机焦磷酸酶处理可减轻磷酸肌酸和分离的抑制化合物的抑制作用；无机焦磷酸盐的抑制作用类似于未纯化的磷酸肌酸；焦磷酸盐存在于商业上可用的磷酸肌酸中，其数量足以解释这种抑制作用。一些商用磷酸肌酸制剂所含焦磷酸盐比其他制剂少得多；这些制剂只有微弱的抑制作用。无机三磷酸是比焦磷酸更有效的酶抑制剂；它也可能作为磷酸肌酸的污染物存在。
Inhibition of rat skeletal muscle adenylate deaminase by creatine phosphate reported previously is due to inorganic pyrophosphate present as a contaminant in commercial preparations of creatine phosphate. This conclusion is based on the following evidence: a compound that inhibits adenylate deaminase can be separated from commercially prepared creatine phosphate by ion exchange chromatography; the inhibition by “creatine phosphate” and by the separated inhibitory compound is relieved by treatment with inorganic pyrophosphatase; inhibition by inorganic pyrophosphate is similar to that produced by unpurified creatine phosphate; and pyrophosphate is present in commercially available creatine phosphate in amounts sufficient to account for the inhibition. Some commercial preparations of creatine phosphate contain much less pyrophosphate than others; these preparations are only weakly inhibitory. Inorganic triphosphate is a more powerful inhibitor of the enzyme than pyrophosphate; it may also be present as a contaminant in creatine phosphate.