A Cell Surface Antigen Expressed on Mouse Lung Macrophages
A Cell Surface Antigen Expressed on Mouse Lung Macrophages
复制标题
小鼠肺巨噬细胞表达的细胞表面抗原
DOI：
10.1111/j.1348-0421.1981.tb00130.x
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发表时间：
1981
影响因子：
2.6
通讯作者：
T. Tokunaga
中科院分区：
文献类型：
--
作者：
K. Akagawa;Y. Maruyama;M. Takano;M. Kasai;T. Tokunaga
Lung macrophages are known to differ from peritoneal macrophages with regard to lysosomal enzymes, metabolic activity for energy requirement, morphology and function (3,6, 13, 14). However, there is no conclusive evidence as to whether or not the lung macrophages belong to a separate subset from peritoneal macrophages. Comparative analysis of the cell surface markers of those macrophages may provide valuable approaches to answer this question. In the first experiment of this study, we tested the binding ability of various lectins onto macrophages from various mouse organs as one of these approaches. Macrophages were harvested from BALB/c mice, C57BL/6 mice (female 6 to 8 week-old, obtained from specific pathogen-free (SPF) colonies of the the Shizuoka Experimental Animal Cooperative, Hamamatsu, Japan), nude mice of BALB/c background (male 6 week-old, bred in our colony) and from germ-free and SPF ICR mice (female 4 week-old, obtained from CLEA Japan Inc., Tokyo, Japan). Macrophages used were obtained from the mice as adherent cells from peritoneal resident cells (PC) harvested by washing out normal peritoneal cavities, peritoneal exudate cells (PEC) harvested from mice received an intraperitoneal injection of proteose peptone 4 days previously, lung cells harvested by chopping up the lung and washing out cells from the cut pieces, or from spleen cells extruded through a 200-mesh stainless steel net and treated with three volumes of 0.5% ammonium chloride to lyse red cells; 30 to 40 ,ul of the PC, PEC, lung cells or splenocytes suspension was spotted on coverslips (6 mm X 10 mm), and incubated at 37 C for 2 hr, then the coverslips were washed to remove nonadherent cells. Usually, more than 96% of the adherent cells of PC, PEC, or lung cells and 65 to 70% of the adherent splenocytes on coverslips ingested more than five latex particles, formed rosettes with IgG-coated sheep erythrocytes and showed macrophage-like morphology. Macrophages on the coverslips were incubated with either of the fluoresceinlabeled peanut agglutinin (FITC-PNA), concanavalin A (FITC-Con A), ricinus agglutinin (FITC-RCA) or Dolichos biflorus agglutinin (FITC-DBA) (KY. Laboratories, Inc., San Mateo, Calif., U.S.A.) for 20 min on ice, and washed. As shown in Table 1 (Exp. A), almost all of both the PC and the lung macrophages were positively stained with FITC-RCA and FITC-Con A, but not FITC-DBA. In contrast, the reactivity of both macrophages to PNA lectin appeared to be quite
糖脂asialo GM1作为胎儿胸腺细胞的新分化抗原。
DOI：
--
发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Habu,S;Kasai,M;Nagai,Y;Tamaoki,N;TAda,T;Herzenberg,LA;Okumura,K
通讯作者：
Okumura,K