trans-10, cis-12 Conjugated Linoleic Acid Enhances In Vitro Maturation of Porcine Oocytes
trans-10, cis-12 Conjugated Linoleic Acid Enhances In Vitro Maturation of Porcine Oocytes
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trans-10、cis-12 共轭亚油酸促进猪卵母细胞的体外成熟
DOI：
10.1002/mrd.22273
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Zhu, Shien
中科院分区：
文献类型：
--
作者：
Jia, Baoyu;Wu, Guoquan;Zhu, Shien
本研究的目的是确定补充反式 10、顺式 12 共轭亚油酸 (t10c12 CLA) 对猪卵母细胞成熟和胚胎发育的影响。与对照相比，在体外成熟（IVM）培养基中添加50μM t10c12 CLA显着增加了中期II（MII）阶段卵母细胞的比例以及随后的孤雌胚胎发育的卵裂率、囊胚形成率和囊胚细胞数量。 t10c12 CLA 处理的卵母细胞恢复减数分裂成熟并进展到 MII 阶段的速度明显快于对照卵母细胞。在 IVM 5、10 和 22 小时时，t10c12 CLA 处理组中卵丘卵母细胞复合体 (COC) 中磷酸化丝裂原激活蛋白激酶 3/1 (p-MAPK3/1) 和环氧合酶 2 (COX2) 的表达显着增加。 t10c12 CLA 处理的 MII 卵母细胞中 p-MAPK3/1 的水平也较高（P
The aim of this study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) supplementation on oocyte maturation and embryo development in pigs. Compared with the control, supplementation of 50 mu M t10c12 CLA to in vitro maturation (IVM) medium significantly increased the proportion of oocytes at the metaphase-II (MII) stage and subsequent parthenogenetic embryo development in terms of cleavage rate, blastocyst formation rate, and cell numbers in blastocysts. The t10c12 CLA-treated oocytes resumed meiotic maturation and progressed to the MII stage significantly faster than those of control. The expression of phosphorylated mitogen-activated protein kinase 3/1 (p-MAPK3/1) and cyclooxygenase-2 (COX2) in cumulus oocyte complexes (COCs) at 5, 10, and 22hr of IVM were significantly increased in the t10c12 CLA-treatment group. The level of p-MAPK3/1 in t10c12 CLA-treated MII oocytes was also higher (P