Prosomatostatin-specific antigen in rat brain: localization by immunocytochemical staining with an antiserum to a synthetic sequence of preprosomatostatin.
Prosomatostatin-specific antigen in rat brain: localization by immunocytochemical staining with an antiserum to a synthetic sequence of preprosomatostatin.
复制标题
大鼠脑中原生长抑素特异性抗原：通过前生长抑素合成序列的抗血清进行免疫细胞化学染色定位。
DOI：
10.1073/pnas.80.9.2780
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Habener,JF
中科院分区：
文献类型：
--
作者：
Lechan,RM;Goodman,RH;Rosenblatt,M;Reichlin,S;Habener,JF
本文用大鼠生长抑素前体（preprosomatostatin）第63-77位氨基酸合成肽（rat somatostatin cryptic peptide，RSCP）的抗血清，比较了免疫反应性RSCP（IR-RSCP）和免疫反应性生长抑素-14（somatostatin-14）在大鼠脑内的分布。IR-RSCP存在于下丘脑tuberoinfundibular系统以及中枢神经系统的不同区域中的神经元细胞体、突起和轴突终末中，与免疫反应性生长抑素呈相同的分布。这些观察结果表明，在神经元中，生长抑素原激素或生长抑素-28的NH 2-末端延伸肽（或两者）与生长抑素14一起在细胞内沿着储存和转运。此外，IR-RSCP在神经末梢的存在表明，这种物质可能作为激素或神经调质分泌，并可作为生长抑素分泌的生物标志物。
Using an antiserum to a 15-amino acid synthetic peptide corresponding to amino acids 63-77 of rat preprosomatostatin (rat somatostatin cryptic peptide, RSCP), we have compared the distribution of immunoreactive RSCP (IR-RSCP) with that of immunoreactive somatostatin-14 in the rat brain. IR-RSCP was present in neuronal cell bodies, processes, and axon terminals in the hypothalamic tuberoinfundibular system as well as in diverse regions of the central nervous system in an identical distribution to immunoreactive somatostatin. These observations indicate that in neurons the somatostatin prohormone or the NH2-terminal extension peptide of somatostatin-28 (or both) is stored and transported intracellularly along with somatostatin 14. In addition, the presence of IR-RSCP in nerve terminals suggests that this material may be secreted as a hormone or neuromodulator and may serve as a biologic marker of somatostatin secretion.