Extraction and analysis of ellagic acid from novel complex sources
Extraction and analysis of ellagic acid from novel complex sources
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DOI：
10.2478/s11696-008-0042-y
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发表时间：
2008-08-01
期刊：
影响因子：
2.2
通讯作者：
Favela-Torres, Ernesto
中科院分区：
文献类型：
--
作者：
Aguilera-Carbo, Antonio F.;Augur, Christopher;Favela-Torres, Ernesto
采用反相高效液相色谱与光电二极管阵列检测相结合的方法，对采自墨西哥半干旱地区的5种细粉植物中的鞣花酸(EA)进行了测定。被分析的样品包括麻疯树树枝(龙血)、大戟树枝(Candelilla)、白花蛇舌草(Damiana)叶(Damiana)、玉米草(Flourensia Cernua)叶(Hojasen)和普尼卡石榴皮(石榴)两个成熟期(“翻身”或中熟果实，视为阳性对照)。结果表明，所有受试样品中的EA浓度都很高，这是这种天然抗氧化剂的新来源。与已报道的EA分析方法相比，该方法具有快速、良好的线性范围、重复性、日内和日间精密度和准确度。
Ellagic acid (EA) was quantified by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detection (DAD) in five fine-powdered plants collected from the semiarid region of Mexico. Samples analysed included Jatropha dioica branches (Dragon's blood), Euphorbia antisyphyllitica branches (Candelilla), Turnera diffusa Willd leaves (Damiana), Flourensia cernua leaves (hojasen) and Punica granatum husk (pomegranate) at two maturity stages ("turning" or intermediate and maturated fruit, considered as positive controls). The results demonstrated high EA concentrations in all tested samples which are novel sources of this natural antioxidant. The method developed for the EA analysis is fast and it showed an excellent linearity range, repeatability, intra-and inter-day precision and accuracy with respect to the methods reported for the EA analysis.