The effects of electric currents on ATP generation, protein synthesis, and membrane transport of rat skin.
The effects of electric currents on ATP generation, protein synthesis, and membrane transport of rat skin.
复制标题
电流对大鼠皮肤 ATP 生成、蛋白质合成和膜运输的影响。
DOI：
10.1097/00003086-198211000-00045
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发表时间：
1982
影响因子：
4.2
通讯作者：
Sansen
中科院分区：
文献类型：
--
作者：
Ngok Cheng;H.J.C. van Hoof;Emmanuel Bockx;Michel J. Hoogmartens;J. C. Mulier;W. Loecker;D. M.;F. J. D. Dijcker;Willy;M.;Sansen
10 微安至 1000 微安的直流电流可增加组织中的 ATP 浓度，并刺激氨基酸掺入大鼠皮肤的蛋白质中。在 100 微安至 750 微安之间刺激氨基酸通过细胞膜的转运，然后是 α-氨基异丁酸的摄取。对 ATP 产生和氨基酸运输的刺激作用显然是由不同的机制介导的，有助于最终增加蛋白质合成活性。在当前应用过程中，胸苷掺入后的 DNA 代谢不受影响。对 AtP 产生的影响可以通过基于米切尔化学渗透理论的质子运动来解释，而运输功能则通过改变跨膜的电梯度来控制。
Direct electric currents ranging from 10 microA to 1000 microA increase ATP concentrations in the tissue and stimulate amino acid incorporation into the proteins of rat skin. The amino acid transport through the cell membrane, followed by the alpha-aminoisobutyric acid uptake, is stimulated between 100 microA and 750 microA. The stimulatory effects on ATP production and on amino acid transport, apparently mediated by different mechanisms, contribute to the final increased protein synthesizing activity. DNA metabolism followed by thymidine incorporation remains unaffected during the course of current application. The effects on AtP production can be explained by proton movements on the basis of the chemiosmotic theory of Mitchell, while the transport functions are controlled by modification in the electrical gradients across the membranes.