Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice
Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice
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DOI：
10.1055/s-0035-1546080
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发表时间：
2015-07-01
期刊：
影响因子：
2.7
通讯作者：
Yuan, Chun-Su
中科院分区：
文献类型：
--
作者：
Li, Hui;Song, Zi-Jing;Yuan, Chun-Su
研究了广东草总黄酮对小鼠乙醇性胃溃疡的抑制作用。用乙醇提取广东黄酮类化合物，用大孔树脂和聚酰胺纯化。采用2,2-二苯基-1-苦味肼基法测定其体外抗氧化活性。采用乙醇性溃疡小鼠模型，研究广东草总黄酮的胃保护作用。此外，还建立了一种方法来保证动物溃疡评价的准确性。在2,2-二苯基-1-苦味酰肼基测定中，广东草总黄酮具有较强的自由基清除能力，IC50为43.83 μ g/mL。在28.16 ~ 112.67 mg/kg剂量范围内，黄酮类化合物显著降低了乙醇诱导小鼠的溃疡指数（p < 0.001）。在胃组织超氧化物歧化酶、过氧化氢酶、谷胱甘肽和髓过氧化物酶水平上，广东草黄酮组与乙醇对照组存在显著差异。广东黄酮类化合物的胃保护作用可能与其抗氧化防御机制有关。结果表明，广东草总黄酮可作为防治胃溃疡的潜在药物。
The present study investigated the flavonoids from Abrus cantoniensis against ethanol-induced gastric ulcers in mice. The flavonoids from A. cantoniensis were extracted with ethanol and purified by macroporous resin and polyamide. The 2,2-diphenyl-1-picrylhydrazyl assay was used to measure the antioxidative activities in vitro. The ethanol-induced ulcer mouse model was used to evaluate the gastroprotective activities of the flavonoids from A. cantoniensis. In addition, a method was established to ensure accuracy for animal ulcer evaluation. The flavonoids from A. cantoniensis showed a strong free radical scavenging capacity with an IC50 of 43.83 mu g/mL in the 2,2-diphenyl-1-picrylhydrazyl assay. At doses between 28.16-112.67 mg/kg, the flavonoids conspicuously reduced the ulcer index in ethanol-induced mice (p < 0.001). Significant differences were found in the levels of superoxide dismutase, catalase, glutathione, and myeloperoxidase in the stomach tissues between the flavonoids from the A. cantoniensis groups and the ethanol control group. The gastroprotective effect of the flavonoids from A. cantoniensis could be due to its antioxidative activity of the defensive mechanism. The data revealed that the flavonoids from A. cantoniensis could be a potential therapeutic agent for gastric ulcer prevention and treatment.