Ultrafast capillary electrophoretic analysis of cereal storage proteins and its applications to protein characterization and cultivar differentiation
Ultrafast capillary electrophoretic analysis of cereal storage proteins and its applications to protein characterization and cultivar differentiation
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DOI：
10.1021/jf990962t
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发表时间：
2000-02-01
影响因子：
6.1
通讯作者：
Lookhart, GL
中科院分区：
文献类型：
--
作者：
Bean, SR;Lookhart, GL
自由区毛细管电泳条件得到了改进，可以快速（2-8分钟）分离几种谷物（小麦、燕麦、水稻、大麦和黑麦）的谷物蛋白质，具有高分辨率和高重复性。该新方法利用等电化合物亚氨基二乙酸（IDA）与20%乙腈和0.05%羟丙基甲基纤维素结合。包括小麦在内的所有谷类品种均可在3 min内通过蛋白或麸质蛋白模式进行分化。与其他酸性缓冲液中分离液的分辨率相似或更高。迁移时间的可重复性非常好，具有运行到运行的可变性
Free zone capillary electrophoresis conditions have been improved to allow rapid (2-8 min) separations of grain proteins from several cereals (wheat, oats, rice, barley, and rye) with high resolution and reproducibility. This new method utilized the isoelectric compound iminodiacetic acid (IDA) in conjunction with 20% acetonitrile and 0.05% hydroxypropylmethylcellulose. Cultivars of all cereals tested could be differentiated in 3 min, including wheat, using either prolamin or glutelin protein patterns. Resolution was similar to or higher than that of separations in other acidic buffers. Migration time repeatability was excellent with run-to-run variability