A novel photoelectrochemical sensor for the detection of α-fetoprotein based on a mesoporous TiO2–CdS QD composite film
A novel photoelectrochemical sensor for the detection of α-fetoprotein based on a mesoporous TiO2–CdS QD composite film
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DOI：
10.1039/c4ay02473g
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发表时间：
2015-02
影响因子：
3.1
通讯作者：
Xiaoyan Yang;Jingyu Li;Junliang Fu
中科院分区：
文献类型：
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作者：
Xiaoyan Yang;Jingyu Li;Junliang Fu
在这篇文章中，一个无标记的光电化学免疫传感器的基础上，CdS修饰的介孔TiO 2薄膜已经开发。通过电化学沉积法将CdS量子点沉积在介孔TiO 2薄膜上，可以提高可见光区的光电流强度。利用抗原-抗体的特异性结合，甲胎蛋白（AFP）可被捕获在修饰电极表面。基于空间位阻效应，光电流强度随AFP浓度的增加而降低。AFP检测的线性范围为0.05 ng mL−1至20 ng mL−1，检测限为0.02 ng mL−1。此外，还对人血清中AFP的实际测定进行了研究。
In this article, a label-free photoelectrochemical immunosensor based on a CdS-modified mesoporous TiO2 film has been developed. The CdS quantum dots could be deposited onto the mesoporous TiO2 film by electrochemical deposition, which could enhance the photocurrent intensity in the visible region. Employing specific binding of antigen–antibody, α-fetoprotein (AFP) could be captured on the surface of the modified electrode. Based on the steric hindrances, the photocurrent intensity decreased with the increase in AFP concentration. A linear response from 0.05 ng mL−1 to 20 ng mL−1 was obtained for AFP detection and the detection limit was 0.02 ng mL−1. Moreover, the practical determination of AFP in human serum was also investigated.