Lamellar--micellar transition of 1-stearoyllysophosphatidylcholine assemblies in excess water.
Lamellar--micellar transition of 1-stearoyllysophosphatidylcholine assemblies in excess water.
复制标题
层状——过量水中 1-硬脂酰溶血磷脂酰胆碱组装体的胶束转变。
DOI：
10.1021/bi00266a035
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Levin,IW
中科院分区：
文献类型：
--
作者：
Wu,W;Huang,C;Conley,TG;Martin,RB;Levin,IW
摘要：虽然人工合成的双酰基磷脂体系经历双层凝胶、液晶或双层六角形相变已有一段时间，但溶血磷脂组件的片层到胶束相转变仅被简要报道[Van Echteld，C.J.，DeKruijff，B.，Mandersloot，J.G.，&DeGier，J.1981)Biochim。生物群落。《学报》649,211-220；黄超，拉皮德斯，J.R.和莱文，I.W.(1982)J.Am化学。(记者发稿)]。本研究应用动态光散射、高灵敏度差示扫描量热法、冷冻断口电子显微镜、31P核磁共振和拉曼光谱技术研究了1-硬脂酰胆碱分散体在过量水中的片层-胶束转变过程。在链熔点以下的温度下，31P核磁共振数据和冷冻断裂电子显微镜结果共同证明了扩展的多层结构的形成。具有链间相互作用特征的拉曼光谱有序/无序指数表明，相反小叶的酰基链可能在片层结构中交错排列。各种物理测量表明
Wen-guey Wu, Ching-hsien Huang,*•* Thomas G. Conley, R. Bruce Martin, and Ira W. Levin* abstract: Although synthetic diacylphospholipid systems have been known for some time to undergo bilayer gel•*-*• liquid crystalline or bilayer* hexagonal phase transitions, lamellar to micellar transitions of lysophospholipid assemblies have only been briefly reported [Van Echteld, C. J. A., DeKruijff, B., Mandersloot, J. G., & DeGier, J.(. 1981) Biochim. Biophys. Acta 649, 211-220; Huang, C., Lapides, J. R., & Levin, I. W.(1982) J. Am. Chem. Soc.(in press)]. In thepresent study we apply dynamic light scattering, high-sensitivity differential scanning calorimetry, freeze-fracture electron microscopy, 31P NMR, and Raman spectroscopic techniques toward investigating the lamellar to micellar transition of 1-stearoyllysophosphatidylcholine dispersions in excess water. At temper-atures below the chain melting transition point, 31P NMR data and freeze-fracture electron microscopic results together demonstrate the formationof extended multilamellar structures. Raman spectroscopic order/disorder indexes, characteristic of interchain interactions, suggest that acylchains of the opposing leaflets are probably interdigitated in the lamellar structures. The various physical measurements indicatethat