INTESTINAL COLONIZATION IN THE CHICKEN BY FOOD-POISONING SALMONELLA SEROTYPES - MICROBIAL CHARACTERISTICS ASSOCIATED WITH FECAL EXCRETION
INTESTINAL COLONIZATION IN THE CHICKEN BY FOOD-POISONING SALMONELLA SEROTYPES - MICROBIAL CHARACTERISTICS ASSOCIATED WITH FECAL EXCRETION
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DOI：
10.1080/03079458808436478
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发表时间：
1988-01-01
期刊：
影响因子：
2.8
通讯作者：
LOVELL, MA
中科院分区：
文献类型：
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作者：
BARROW, PA;SIMPSON, JM;LOVELL, MA
经口接种新孵化或三周龄的鸡后，沙门氏菌在盲肠中的存活时间最长，在鸡体内的存活时间也较短。S. cholerae-suis、猪霍乱肠杆菌E.大肠杆菌K12和酿酒酵母没有殖民的消化道时，口服接种，但他们坚持在盲肠比在其他地区的肠道。这表明盲肠中沙门氏菌的优先定位可能是非特异性或宿主因素的结果。这可能包括内容物通过该器官的缓慢流动速率，这将允许更大的微生物繁殖和持久性。一些沙门氏菌菌株，特别是S.经口接种到3周龄鸡体内的鼠伤寒杆菌，与从这些器官的腔内容物中分离出的鼠伤寒杆菌相比，从盲肠壁的匀浆中分离出的鼠伤寒杆菌数量更多，而从盲肠壁分离出的鼠伤寒杆菌数量少得多。没有一种菌株与作物壁的结合程度与乳酸杆菌相同。在口服接种食物中毒沙门氏菌血清型后，在至少4周的时间内，在其粪便中排泄沙门氏菌的鸡的数量逐渐下降。相反，宿主适应的血清型如S. cholerae-suis和S.羊流产病毒的排泄时间不超过几天。通过对S.鼠伤寒沙门氏菌和S.结果表明，该菌的鞭毛（H）抗原、体细胞（O）抗原、甘露糖敏感血凝素和85-糖苷酶质粒均不具有易识别的特征。鼠伤寒沙门氏菌，是必不可少的殖民（持续粪便排泄）。然而，S.由亚硝基胍处理产生的细菌仍然具有鞭毛和体细胞抗原以及血凝素，但没有定殖消化道，这表明一种未鉴定的微生物因子是定殖所必需的。
Following oral inoculation of newly-hatched or three-week-old chickens, Salmonella organisms persisted longest in the caeca and they were also present for a shorter time in the crop. S. cholerae-suis, E. coli K12 and Saccharomyces cerevisae did not colonise the alimentary tract when inoculated orally, but they persisted longer in the caeca than in other regions of the gut. This suggested that preferential localisation of Salmonella in the caeca may be the result of non-specific or host factors. This may include the slow rate of flow of contents through this organ which would allow greater microbial multiplication and persistence. Some Salmonella strains, particularly S. typhimurium inoculated orally into three-week-old chickens were isolated in greater numbers from homogenates of the crop wall and, much less frequently, from the caecal wall, than from the luminal contents of these organs. None of the strains associated with the crop wall to the same extent as did lactobacilli. After oral inoculation of food-poisoning Salmonella serotypes the number of chickens excreting Salmonellae in their faeces gradually declined over a period of at least 4 weeks. In contrast, host adapted serotypes such as S. cholerae-suis and S. abortus-ovis were not excreted for longer than a few days. By testing mutants of S. typhimurium and S. infantis which were devoid of easily recognised characteristics it was concluded that neither flagellar (H) antigens, somatic (O) antigens, mannose-sensitive haemagglutinins nor the possession of the virulence associated 85 kilobase plasmid of S. typhimurium, were essential for colonisation (persistent faecal excretion). However, a mutant of S. infantis which was created by nitroso-guanidine treatment still possessed flagellar and somatic antigens and haemagglutinins and yet did not colonise the alimenatry tract, indicating that an unidentified microbial factor was essential for colonisation.