Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Expression of non-neuronal cholinergic system in maxilla of rat in vivo
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DOI：
10.1186/0717-6287-47-72
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发表时间：
2014-01-01
影响因子：
6.7
通讯作者：
Che, Xiaoxia
中科院分区：
文献类型：
--
作者：
Guo, Jie;Wang, Lue;Che, Xiaoxia
背景资料：乙酰胆碱（Acetylcholine，ACh）是中枢和外周神经系统中的一种重要神经递质，也存在于多种非神经组织和细胞中。ACh在上颌骨中的存在及其对成骨的潜在调控作用有待进一步研究。结果如下：胆碱能系统的组成部分采用Real-Time PCR和免疫组织化学方法检测了大鼠上颌骨乙酰胆碱（Ach）、酯酶（esterase）、胆碱乙酰转移酶（choline acetyltransferase）、高亲和力胆碱摄取（high-affinity choline uptake）、N-乙酰胆碱受体（n-AChRs）和mAChRs的表达（Slc 25 a20）、VAChT、OCTN 2、OCT 1、OCT 3、有机阳离子转运蛋白成员4（Slc 22 a4）、AChE、BChE、nAChR亚基α 1、α 2、α 3、α 5、α 7、α 10、β 1、β 2、β 4、结果：（1）在大鼠上颌骨中检测到γ和mAChR亚单位M1、M2、M3、M4、M5。VAChT、AChE、nAChR α 2、β 1、β 4亚基和mAChR M4亚基的RNA表达丰富（2（-Δ Ct）> 0.03）。对ACh、VAChT、nAChR α 7和AChE进行免疫组织化学染色。ACh在间充质细胞、成软骨细胞、骨和软骨基质及骨髓细胞中均有表达，VAChT表达广泛，ACh受体α 7在软骨内新形成的骨基质及骨髓骨化中强表达，AchE仅在间充质干细胞、软骨及骨髓细胞中表达。ACh可能通过影响上颌骨的软骨内成骨、骨髓成骨和骨基质矿化而发挥作用。
Background: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. Results: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry.Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits alpha 1, alpha 2, alpha 3, alpha 5, alpha 7, alpha 10, beta 1, beta 2, beta 4, gamma and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits alpha 2, beta 1, beta 4 and mAChR subunits M4 had abundant expression (2(-Delta Ct) > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChR alpha 7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor a7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells.Conclusions: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.