1 Alpha-25,26-trihydroxyvitamin D3: an in vivo and in vitro metabolite of vitamin D3.
1 Alpha-25,26-trihydroxyvitamin D3: an in vivo and in vitro metabolite of vitamin D3.
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1 Alpha-25,26-三羟基维生素 D3：维生素 D3 的体内和体外代谢物。
DOI：
10.1021/bi00524a050
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Horst,RL
中科院分区：
文献类型：
--
作者：
Reinhardt,TA;Napoli,JL;Praminik,B;Littledike,ET;Beitz,DC;Partridge,JJ;Uskoković,MR;Horst,RL
材料和方法总则。高压液相色谱在LC-204型高效液相色谱仪(Waters Associates，Milford，MA)上进行。2用Finnigan自动EI/CI，GC/MS与INCOS数据系统相连接的固体探针获得了质谱图。分析在70 eV下进行，电离器温度为250℃，同时将探针从环境加热到320℃，在乙醇中使用Beckman DB记录光谱仪获得紫外光谱。泽西州的奶牛按照描述用维生素D3处理(Horst&Littledike，1979)。治疗5周后，采血于肝素化容器中。血浆用离心法制备，在-20℃下保存，直至提取。
Materials and MethodsGeneral. High-pressure liquid chromatography was per-formed on a Model LC-204 HPLC (Waters Associates, Milford, MA). 2 Mass spectra were obtained with the solids probe of a Finnigan automated EI/CI, GC/MS system cou-pled to an INCOS data system. Analyses were performed at 70 eV with an ionizer temperature of 250 C while the probe was heated from ambient to320 C. Ultraviolet spectra were obtained in ethanol by using a Beckman DB recording spec-trophotometer.Animals. Jersey cows were treated with vitamin D3 as described (Horst & Littledike, 1979). After 5 weeks of treatment, blood was collected in heparinized containers. Plasma was prepared by centrifugation and stored at-20 C until extracted.