Effect of hyperoxia on the cytoarchitecture of cultured endothelial cells.
Effect of hyperoxia on the cytoarchitecture of cultured endothelial cells.
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发表时间：
1988
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通讯作者：
P. Phillips;P. Higgins;A. Malik;M. Tsan
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作者：
P. Phillips;P. Higgins;A. Malik;M. Tsan
当培养的肺动脉内皮细胞融合暴露于高氧（95%O2和5%CO2），他们变得扩大和平均红细胞体积增加30- 35%。肌动蛋白丝的罗丹明鬼笔环肽染色表明，高氧与肌动蛋白分布的进行性改变有关。氧暴露后3天，细胞质应力纤维的数量和厚度增加，而周围带被破坏或缺失。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，在氧暴露的细胞中的丝状肌动蛋白的量增加，而总的肌动蛋白含量保持不变，表明氧暴露的平衡从G肌动蛋白转移到F肌动蛋白。
When confluent pulmonary artery endothelial cells in culture were exposed to hyperoxia (95% O2 and 5% CO2), they became enlarged and mean corpuscular volume increased 30-35%. Rhodamine-phalloidin staining of actin filaments demonstrated that hyperoxia was associated with a progressive alteration in the actin distribution. Three days after oxygen exposure, the number and thickness of cytoplasmic stress fibers were increased, while the peripheral bands were disrupted or absent. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the amount of filamentous actin was increased in oxygen-exposed cells, while the total actin content remained unchanged, suggesting that oxygen exposure shifted the equilibrium from G actin to F actin.