Structural and functional alterations in the rat lung following whole thoracic irradiation with moderate doses: Injury and recovery
Structural and functional alterations in the rat lung following whole thoracic irradiation with moderate doses: Injury and recovery
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DOI：
10.1080/09553000802078396
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Medhora, Meetha
中科院分区：
文献类型：
--
作者：
Zhang, Rong;Ghosh, Swarajit N.;Medhora, Meetha
目的：为了表征结构和功能损伤后，一个单一的剂量的全胸部照射，可能是存活后的核attack/accident.Methods：大鼠暴露于5或10戈伊的X射线与其他器官屏蔽的整个胸部。非侵入性的呼吸率和动脉血氧饱和度的测量，和侵入性的支气管肺泡灌洗液，（总蛋白，克拉拉细胞分泌蛋白），血管反应性和组织学进行了至少6个时间点到52 weeks after irradiation.Results：10戈伊的照射导致呼吸率增加，氧饱和度减少，支气管肺泡灌洗液中蛋白的增加和衰减的血管反应性之间的4-12周照射后。在5戈伊的低剂量下未观察到这些变化。组织学检查显示，两种剂量照射后4-8周血管周围水肿，10戈伊照射后20周以上出现轻度肺纤维化。结论：10而非5戈伊X线照射可导致大鼠胸部氧摄取和血管反应性降低，呼吸频率增加，提示早期肺血管病变。暴露后20周，血管水肿消退，并被轻度纤维化取代，同时肺功能恢复。
Purpose:To characterize structural and functional injuries following a single dose of whole-thorax irradiation that might be survivable after a nuclear attack/accident.Methods:Rats were exposed to 5 or 10 Gy of X-rays to the whole thorax with other organs shielded. Non-invasive measurements of breathing rate and arterial oxygen saturation, and invasive evaluations of bronchoalveolar lavage fluid, (for total protein, Clara cell secretory protein), vascular reactivity and histology were conducted for at least 6 time points up to 52 weeks after irradiation.Results:Irradiation with 10 Gy resulted in increased breathing rate, a reduction in oxygen saturation, an increase in bronchoalveolar lavage fluid protein and attenuation of vascular reactivity between 4-12 weeks after irradiation. These changes were not observed with the lower dose of 5 Gy. Histological examination revealed perivascular edema at 4-8 weeks after exposure to both doses, and mild fibrosis beyond 20 weeks after 10 Gy.Conclusions:Single-dose exposure of rat thorax to 10 but not 5 Gy X-irradiation resulted in a decrease in oxygen uptake and vasoreactivity and an increase in respiratory rate, which paralleled early pulmonary vascular pathology. Vascular edema resolved and was replaced by mild fibrosis beyond 20 weeks after exposure, while lung function recovered.