A Method for Obtaining Infective Nematode Larvae from Cultures
A Method for Obtaining Infective Nematode Larvae from Cultures
复制标题
从培养物中获得感染性线虫幼虫的方法
DOI：
10.1126/science.66.1709.302.b
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发表时间：
1927
期刊：
影响因子：
56.9
通讯作者：
G. F. White
中科院分区：
文献类型：
--
作者：
G. F. White
Fig. 1.用于培养线虫幼虫的装置。a，结晶皿; B，含炭粪混合物的皮氏培养皿; o，表面玻璃盖。水包围着皮氏培养皿，约等于其深度的一半。培养物的温育优选在可保持高湿度的地方。许多接近第三幼虫期的幼虫从培养物中迁移出来，并被困在培养皿周围的水中。在收集它们的过程中，移开表面玻璃盖，最好用镊子将装有活性炭培养物的半个皮氏培养皿取出并放入其中，将含有活性炭的水从结晶培养皿倒入试管中，然后将试管垂直放置在架子上。蠕虫很快便会孕至管底，然后用移液管吸去管底的水分，使幼虫浓缩。然后，可以将具有木炭混合物的设备重新组装并通过蒸汽灭菌。可以对设备和方法进行许多修改以满足工人的特殊需要。当只有少量培养物时，最好使用半个培养皿，底部朝上。一个锡拉丘兹的观察玻璃或科普利亚罐的顶部可以很好地代替皮氏培养皿。室温，特别是在夏天，可以代替恒温箱。一种改进的装置已被用来在需要时稍微减少培养物中真菌的生长量。一个直径与结晶盘相同的铝盘，其倾斜的一面被穿孔，放置在表面玻璃盖的下方，并由盘的边缘支撑。往锅里倒入几立方厘米的福尔马林水溶液。15%的溶液已成功使用，但最佳强度应由工人根据自己的需要确定。D
FIG. 1. Apparatus used for culturing nematode larvae. a, Crystallizing dish; b, Petri dish with charcoal-feces mixture; o, watch-glass cover. Water surrounds the Petri dish equal to about one half its depth. incubation of the culture preferably where a high humidity can be maintained. Many of the larvae on approaching the third larval stage migrate from the culture and are trapped in the water surrounding the Petri dish. In collecting them the watch-glass cover is removed and the half Petri dish with the charcoal culture is lifted out, preferably with forceps, and placed into it. The water containing the rarvae is poured from the crys-tallizing dish into a test-tube which is then stood perpendicularly in the rack. The worms soon gravi-tate to the bottom of the tube, after which the watex above may be pipetted off, leaving the larvae con-centrated. The apparatus with the charcoal mixture may then be reassembled and sterilized by steaming A number of modifications of the apparatus and the method may be made to meet the worker's special needs. When there is but a small amount of culture it is well to use the half Petri dish with the bottom up. A Syracuse watch-glass or the top of a Coplia jar serves well in place of the Petri dish. Room temperature, especially in summer, may be substitutec for the more constant one of an incubator. A modified form of the apparatus has been used tc reduce somewhat the amount of fungous growth ix the culture when this seemed desirable. An alumi num pan of the diameter of the crystalling dish with the inclined side perforated, is placed beneati the watch-glass cover and supported by the edge oi the dish. Into the pan is poured a few cubic centi meters of an aqueous solution of formalin. A 15 pe: cent. solution has been employed successfully, but thi optimum strength should be determined by eadl worker to meethis own needs. d