Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos
Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos
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DOI：
10.1016/j.ygeno.2007.09.001
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发表时间：
2008-02-01
期刊：
影响因子：
4.4
通讯作者：
Palma, Veronica
中科院分区：
文献类型：
--
作者：
Bergeron, Sadie A.;Milla, Luis A.;Palma, Veronica
Hedgehog（Hh）信号通路在胚胎发育过程中起着重要的指导作用。Hh/Gli信号传导的失调是人类先天性疾病和各种癌症的主要致病因素。斑马鱼是研究胚胎发育过程中Hh信号传导的强大遗传模型，因为已经鉴定了大量影响Hh/Gli信号传导系统不同组分的突变体。通过在不同的Hh/Gli功能获得和丧失情况下进行基因表达的全局分析，我们鉴定了已知的（例如，ptc 1和nkx2.2a）和在具有改变的Hh/Gh信号传导功能的胚胎中差异表达的新型Hh调节基因。通过揭示组织特异性基因表达的变化，我们揭示了受Hh信号影响的新的胚胎学过程。因此，我们提供了一个全面的调查Hh/GLI调控基因在胚胎发生过程中，我们确定新的Hh调控基因，可能是在肿瘤发生过程中的失调的目标。(c)2007年爱思唯尔公司All rights reserved.
The Hedgehog (Hh) signaling pathway plays critical instructional roles during embryonic development. Misregulation of Hh/Gli signaling is a major causative factor in human congenital disorders and in a variety of cancers. The zebrafish is a powerful genetic model for the study of Hh signaling during embryogenesis, as a large, number Of Mutants that affect different components of the Hh/Gli signaling system have been identified. By performing global profiling of gene expression in different Hh/Gli gain- and loss-of-function scenarios we identified known (e.g., ptc1 and nkx2.2a) and novel Hh-regulated genes that are differentially expressed in embryos with altered Hh/Gh signaling function. By uncovering changes in tissue-specific gene expression, we revealed new embryological processes that are influenced by Hh signaling. We thus provide a comprehensive survey of Hh/Gli-regulated genes during embryogenesis and we identify new Hh-regulated genes that may be targets of misregulation during tumorigenesis. (c) 2007 Elsevier Inc. All rights reserved.