Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice.
Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice.
复制标题
水稻COPT/Ctr型铜转运蛋白样基因家族的分子和功能分析
DOI：
10.1186/1471-2229-11-69
复制
发表时间：
2011-04-21
影响因子：
5.3
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Yuan M;Li X;Xiao J;Wang S
背景铜转运蛋白（COPT/Ctr）基因家族在维持不同物种的铜稳态中起重要作用。水稻COPT型基因家族由7个成员（COPT 1至COPT 7）组成。然而，只有两个，COPT 1和COPT 5，已被其功能的特点是在铜transport.ResultsHere，我们报告的其他五个成员的riceCOPT基因家族（COPT 2，COPT 3，COPT 4，COPT 6，和COPT 7）的分子和功能特性。水稻COPT家族的所有成员都具有已知的COPT/Ctr型铜转运蛋白基因的保守特征。在水稻COPT编码的蛋白质中，除了已知的COPT 1和COPT 5之间的相互作用外，COPT 2、COPT 3和COPT 4分别与COPT 6发生物理相互作用。COPT 2、COPT 3或COPT 4与COPT 6协同作用，在缺乏ScCtr 1和ScCtr 3的酵母菌中介导了高亲和力的Cu吸收。COPT 7单独可以介导酵母突变体中的高亲和力Cu摄取。7种COPT单独或联合应用均不能与S型互补。缺乏铁吸收或锌吸收转运基因的突变体。然而，theseCOPT基因，表现出不同的组织特异性表达模式和铜水平调控的表达模式，也转录的影响，由缺乏铁，锰，或zinch.ConclusionThese结果表明，COPT 2，COPT 3，和COPT 4可能与COPT 6，分别和COPT 7单独行动铜运输在不同的水稻组织。铁、锰、锌的内源浓度可能通过影响COPT基因的表达而影响铜的稳态。
BackgroundThe copper (Cu) transporter (COPT/Ctr) gene family has an important role in the maintenance of Cu homeostasis in different species. The rice COPT-type gene family consists of seven members (COPT1toCOPT7). However, only two,COPT1andCOPT5, have been characterized for their functions in Cu transport.ResultsHere we report the molecular and functional characterization of the other five members of the riceCOPTgene family (COPT2,COPT3,COPT4,COPT6, andCOPT7). All members of the riceCOPTfamily have the conserved features of knownCOPT/Ctr-type Cu transporter genes. Among the proteins encoded by riceCOPTs, COPT2, COPT3, and COPT4 physically interacted with COPT6, respectively, except for the known interaction between COPT1 and COPT5. COPT2, COPT3, or COPT4 cooperating with COPT6 mediated a high-affinity Cu uptake in the yeastSaccharomyces cerevisiaemutant that lacked the functions of ScCtr1 and ScCtr3 for Cu uptake. COPT7 alone could mediate a high-affinity Cu uptake in the yeast mutant. None of the seven COPTs alone or in cooperation could complement the phenotypes ofS. cerevisiaemutants that lacked the transporter genes either for iron uptake or for zinc uptake. However, theseCOPTgenes, which showed different tissue-specific expression patterns and Cu level-regulated expression patterns, were also transcriptionally influenced by deficiency of iron, manganese, or zinc.ConclusionThese results suggest that COPT2, COPT3, and COPT4 may cooperate with COPT6, respectively, and COPT7 acts alone for Cu transport in different rice tissues. The endogenous concentrations of iron, manganese, or zinc may influence Cu homeostasis by influencing the expression ofCOPTsin rice.
登录
查看更多内容
影响因子：
3.7
作者：
Ioannoni R;Beaudoin J;Mercier A;Labbé S
通讯作者：
Labbé S
影响因子：
5.3
作者：
Peña, MMO;Koch, KA;Thiele, DJ
通讯作者：
Thiele, DJ
影响因子：
4.5
作者：
Petris, MJ
通讯作者：
Petris, MJ
影响因子：
2.8
作者：
Marvin, ME;Mason, RP;Cashmore, AM
通讯作者：
Cashmore, AM
影响因子：
4.8
作者：
Lee, J;Peña, MMO;Thiele, DJ
通讯作者：
Thiele, DJ