LEAD SPECIATION BY HPLC-ICP-AES AND HPLC-ICP-MS
LEAD SPECIATION BY HPLC-ICP-AES AND HPLC-ICP-MS
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DOI：
10.1093/chromsci/29.3.98
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发表时间：
1991-03-01
影响因子：
1.3
通讯作者：
CARUSO, JA
中科院分区：
文献类型：
--
作者：
ALRASHDAN, A;HEITKEMPER, D;CARUSO, JA
利用电感耦合等离子体发射光谱(ICPAES)和电感耦合等离子体质谱(ICPMS)对无机铅(Pb2+)和几种三烷基铅形态(三甲基氯化铅[TML]、三乙基氯化铅[TEL]和三苯基氯化铅[TPhL])的形态进行了研究。研究了反相、离子配对和离子交换高效液相色谱模式。用于电感耦合等离子体原子发射光谱分析的最佳色谱条件包括采用10%至70%甲醇梯度的反相分离。然而，在使用电感耦合等离子体质谱检测时，已经发现梯度会使血浆不稳定。用30%甲醇为流动相的等度分离是等离子体稳定性和色层分辨率之间的最佳折衷。电感耦合等离子体质谱检测的检出限比电感耦合等离子体原子发射光谱分析提高了3个数量级。
Speciation of inorganic lead (Pb2+) and several trialkyllead species (trimethyllead chloride [TML], triethyllead chloride [TEL], and triphenyllead chloride [TPhL]) is investigated using high-performance liquid chromatography (HPLC) with detection by both inductively coupled plasma emission spectroscopy (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS). Reversed-phase, ion-pairing, and ion-exchange HPLC modes are studied. Optimal chromatographic conditions for ICP-AES detection include a reversed-phase separation utilizing a step gradient from 10 to 70% methanol. However, the gradient has been found to destabilize the plasma when using ICP-MS detection. An isocratic separation with a 30% methanol mobile phase has been found to be the best compromise between plasma stability and chromatographic resolution. Detection limits using ICP-MS detection are 3 orders of magnitude improved over ICP-AES detection.