Ischemia-reperfusion injury of the mouse kidney.
Ischemia-reperfusion injury of the mouse kidney.
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DOI：
10.1007/978-1-61779-851-1_32
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
de Caestecker, Mark P
中科院分区：
文献类型：
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作者：
Oxburgh, Leif;de Caestecker, Mark P
对肾脏对急性损伤的复杂反应的研究对组织损伤和修复机制产生了重要的见解。各种损伤模型为这一令人印象深刻的知识体系做出了贡献，但缺血再灌注（IR）模型可能是使用最广泛的。本章包含小鼠 IR 损伤的详细方法描述以及血液采样和组织采集的注释。本章的目的是为新手提供在其研究计划中建立此程序的分步指南。
Studies of the complex responses of the kidney to acute injury have yielded important insights into mechanisms of tissue injury and repair. A variety of injury models have contributed to this impressive body of knowledge, but the ischemia-reperfusion (IR) model has perhaps been the most widely used. This chapter contains a detailed method description for IR injury in the mouse together with notes on blood sampling and tissue harvesting. The aim of the chapter is to provide the novice with a step-by-step guide to establishing this procedure in their research program.