Fish rhabdovirus cell entry is mediated by fibronectin
Fish rhabdovirus cell entry is mediated by fibronectin
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DOI：
10.1128/jvi.73.9.7703-7709.1999
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Bremont, M
中科院分区：
文献类型：
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作者：
Bearzotti, M;Delmas, B;Bremont, M
针对虹鳟性腺细胞（RTG-2）产生的三种单克隆抗体（mab）已被选择，因为它们能够保护细胞免受病毒性出血性败血症病毒（VHSV）感染，这是一种鲑科横纹肌病毒。对感染的保护仅限于鲑鱼来源的细胞系：表明阻断单克隆抗体的物种特异性。令人惊讶的是，这些单克隆抗体的阻断活性对其他非抗原性相关的鱼横纹肌病毒也有效。间接免疫荧光和免疫电镜观察表明，这三种单抗均针对丰富的细胞质膜成分，免疫沉淀研究表明，靶标由异二聚体复合物组成，分子量为200和44 kDa。生化数据提供了以下证据，证明纤维连接蛋白是该复合体的一部分，它可能代表鱼类横纹肌病毒的主要受体。(i)生成的抗200 kda蛋白的抗血清与大肠杆菌中表达的重组虹鳟鱼纤维连接蛋白发生反应。（ii）纯化的虹鳟鱼纤维连接蛋白能够与VHSV特异性结合。据我们所知，这是a的第一次鉴定；作为低等脊椎动物中病毒复制的主要受体的细胞成分，更有趣的是，它也是横纹肌病毒科病毒的主要受体。
Three monoclonal antibodies (MAbs) generated against rainbow trout gonad cells (RTG-2) have been selected for their ability to protect cells from the viral hemorrhagic septicemia virus (VHSV) infection, a salmonid rhabdovirus. Protection from infection was restricted to the salmonid-derived cell lines: indicating species specificity of the blocking MAbs. Surprisingly, the blocking activity of these MAbs was also effective against other nonantigenically related fish rhabdoviruses. Indirect immunofluorescence and immunoelectron microscopy observations demonstrated that the three MAbs were all directed against an abundant cell plasma membrane component, and immunoprecipitation studies indicated that the target consisted of a heterodimeric complex with molecular masses of 200 and 44 kDa. Biochemical data provided the following evidence that fibronectin is part of this complex and that it could represent the main receptor for fish rhabdoviruses. (i) An antiserum generated against the 200-kDa protein reacted against the recombinant rainbow trout fibronectin expressed in Escherichia coli. (ii) The purified rainbow trout fibronectin was able to bind specificality to VHSV. To our knowledge, this is the first identification of a;cellular component acting as a primary receptor for a virus replicating in lower vertebrates and, more interestingly, for viruses belonging to the Rhabdoviridae family.