TNF induces distinct gene expression programs in microvascular and macrovascular human endothelial cells
TNF induces distinct gene expression programs in microvascular and macrovascular human endothelial cells
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DOI：
10.1189/jlb.0905530
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发表时间：
2006-07-01
影响因子：
5.5
通讯作者：
Roth, Johannes
中科院分区：
文献类型：
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作者：
Viemann, Dorothee;Goebeler, Matthias;Roth, Johannes
内皮细胞（EC）多样性与炎症刺激反应的相关性目前尚未明确。利用寡核苷酸微阵列技术，我们系统地分析了人微血管内皮细胞（HMEC）和大血管人脐静脉内皮细胞（HUVEC）中肿瘤坏死因子（TNF）诱导的表达谱，通过微阵列分析分析了13,000个人类基因。使用严格的纳入和排除标准，微阵列分析显示大约一半的 TNF 诱导基因对 HMEC-1 或 HUVEC 具有特异性。微阵列数据可以通过定量逆转录酶-聚合酶链反应和蛋白质水平得到广泛证实。有趣的是，大多数这些基因的调节取决于编码趋化因子、细胞因子和细胞表面分子的细胞类型。我们的结果表明需要更仔细地考虑仅限于 EC 不同亚型的具体影响。因此，EC类型特异性表达模式的建立可能为选择性操纵不同炎症性疾病中特定内皮亚型提供基础。
The relevance of the diversity of endothelial cells (ECs) for the response to inflammatory stimuli is currently not well defined. Using oligonucleotide microarray technique, we systematically analyzed the tumor necrosis factor (TNF)-induced expression profile in human microvascular ECs (HMEC) and macrovascular human umbilical vein ECs (HUVEC), analyzing 13,000 human genes by microarray analysis. Using strict inclusion and exclusion criteria, microarray analysis revealed that about half of the TNF-induced genes were specific for HMEC-1 or HUVEC. The microarray data could widely be confirmed by quantitative reverse transcriptase-polymerase chain reaction and at the protein level. It is interesting that the majority of those genes regulated depending on the cell type encoded for chemokines, cytokines, and cell surface molecules. Our results argue for a more careful consideration of specific effects restricted to distinct subtypes of ECs. The establishment of EC type-specific expression patterns may thus provide the basis for a selective manipulation of specific endothelial subtypes in different inflammatory diseases.