Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients
Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients
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DOI：
10.1016/j.fertnstert.2008.01.086
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发表时间：
2009-05-01
影响因子：
6.7
通讯作者：
Benkhalifa, Moncef
中科院分区：
文献类型：
--
作者：
Cohen-Bacrie, Paul;Belloc, Stephanie;Benkhalifa, Moncef
目的：利用末端脱氧核糖核苷转移酶介导的dUTP镍端标记技术，研究精子中DNA片段化与世界卫生组织参数和计算机辅助精子分析（CASA）的关系，探讨获得CASA参数、精子形态和DNA片段化之间相关性的可能性。设计：根据世界卫生组织参数进行精子分析，末端脱氧核糖核苷酸转移酶介导的dUTP镍端标记（TUNEL）用于精子DNA片段化，CASA用于精子运动。前瞻性研究。环境：所有患者都在临床管理下，在法国生育中心咨询不孕问题。患者：一千六百三十三名男性接受不孕症调查，包括完整的精子分析。干预措施：精子分析和DNA损伤检测。主要观察指标：精子形态、DNA片段和运动特征。结果：1 / 3的患者TUNEL发生率为30%。对21个精液参数的分析显示，其中7个与TUNEL结果显著相关。结论：世界卫生组织精子参数和DNA损伤是互补的，而不是紧密相关的。应该考虑到这一点，以便更充分地了解父亲在辅助生殖技术失败中的作用。[j] .中国农业科学（英文版），2009;31(1):391 - 391。(C) 2009年美国生殖医学学会。)
Objective: To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation.Design: Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study.Setting: All the patients were under clinical management, consulting for hypofertility at a fertility center in France.Patient(s): One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis.Intervention(s): Sperm analysis and DNA damage testing.Main Outcome Measure(s): Sperm morphology, DNA fragmentation, and movement characteristics.Result(s): One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results. Conclusion(s): World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures. (Fertil Steril(R) 2009;91:1801-5. (C) 2009 by American Society for Reproductive Medicine.)