Overexpression of phosphorylated-STAT3 correlated with the invasion and metastasis of cutaneous squamous cell carcinoma
Overexpression of phosphorylated-STAT3 correlated with the invasion and metastasis of cutaneous squamous cell carcinoma
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DOI：
10.1111/j.1346-8138.2005.tb00906.x
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发表时间：
2005-05-01
影响因子：
3.1
通讯作者：
Chen, LR
中科院分区：
文献类型：
--
作者：
Cai, SQ;Zheng, M;Chen, LR
为探讨STAT3磷酸化和E-钙粘蛋白表达在某些人表皮非黑色素瘤皮肤肿瘤转移中的可能作用，采用免疫组织化学方法检测了皮肤鳞状细胞癌(SCC)、基底细胞癌(BCC)和脂溢性角化病(SK)石蜡包埋切片中磷酸化STAT3(p-STAT3)和E-钙粘附素的表达。应用免疫组织化学方法检测30例皮肤鳞状细胞癌、20例基底细胞癌、20例SK和20例正常皮肤组织中p-STAT3和E-cadherin蛋白的表达。结果表明：1)鳞状细胞癌组织中p-STAT3蛋白异常升高，与正常皮肤和SK相比差异有统计学意义(p<；0.001)。P-STAT3在鳞状细胞癌中的表达也显著高于基底细胞癌(p<；0.05)。2)p-STAT3在低分化鳞癌中的表达高于高分化鳞癌(p<；0.05)。P-STAT3的阳性表达与肿瘤的侵袭深度和有无转移密切相关(p<；0.05)，而与肿瘤大小无关。3)E-钙粘附素在正常皮肤和SK的细胞膜上呈强阳性表达，尤以基底细胞表达最强。E-钙粘附素在鳞状细胞癌和基底细胞癌细胞膜上的表达较弱(p<；0.001)，而在鳞状细胞癌中的表达明显低于基底细胞癌(p<；0.05)。在鳞癌中，E-钙粘附素的表达强度与肿瘤的分化程度有关，而与肿瘤的侵袭深度和肿瘤大小无关。4)p-STAT3和E-钙粘素在鳞癌中的表达强度呈负相关(r(X)=-0.372，p<；0.05)。我们认为p-STAT3的过表达可能在表皮肿瘤的发生发展中起重要作用。STAT3的异常激活可能与SCC的转移潜能有关，联合检测p-STAT3和E-cadherin可能有助于预测SCC的预后。
In order to evaluate the possible effects of STAT3 phosphorylation and expression of E-cadherin on metastasis of some human epidermal non-melanoma cutaneous tumors, the expression of phosphorylated STAT3 (p-STAT3) and E-cadherin were analyzed by immunohistochemistry staining in formalin-fixed, paraffin-embedded tissue sections of human cutaneous squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and seborrhoeic keratosis (SK). An immunohistochemistry staining technique was employed to measure the expression of p-STAT3 and E-cadherin protein in 30 cases of cutaneous SCC, 20 cases of BCC, 20 cases of SK, and 20 specimens of normal skin. The results were as follows: 1) p-STAT3 protein was abnormally increased in SCC as compared to normal skin and SK (p < 0.001). Expression of p-STAT3 in SCC was also significantly higher than in BCC (p < 0.05). 2) Expression of p-STAT3 was higher in poorly-differentiated SCC than in well-differentiated ones (p < 0.05). The positive rate of the expression of p-STAT3 correlated well with the depth of tumor invasion and with metastasis (p < 0.05), but there was no correlation between the positive rate and tumor size. 3) E-cadherin was strongly expressed on the cell membranes of normal skin and SK, especially on basal cells. E-cadherin was weakly expressed on cell membranes of SCC and BCC (p < 0.001), whereas its expression was significantly lower in SCC than in BCC (p < 0.05). In SCC, the intensity of E-cadherin expression was correlated with the extent of tumor differentiation, but there was no correlation between the expression intensity and the depth of tumor invasion or tumor size. 4) There was a negative correlation between the expression intensity of p-STAT3 and E-cadherin in SCC (r(x) = -0.372, p < 0.05). We concluded that the overexpression of p-STAT3 may have an important role in the development of epidermal tumors. Abnormal activation of STAT3 may be related to metastasis potential in SCC and the simultaneous detection of p-STAT3 and E-cadherin may contribute to predicating the prognosis in SCC.