Nicotine and cotinine affect the release of vasoactive factors by trophoblast cells and human umbilical vein endothelial cells
Nicotine and cotinine affect the release of vasoactive factors by trophoblast cells and human umbilical vein endothelial cells
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DOI：
10.1016/j.placenta.2010.11.010
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发表时间：
2011-02-01
期刊：
影响因子：
3.8
通讯作者：
Apa, R.
中科院分区：
文献类型：
--
作者：
Romani, F.;Lanzone, A.;Apa, R.
目的：研究尼古丁（N）和可替宁（C）对滋养层细胞（TC）和人脐静脉内皮细胞（HUVEC）分泌可溶性fms样酪氨酸激酶（sFlt-1）、可溶性内皮糖蛋白（sENG）、胎盘生长因子（PIGF）、转化生长因子-β（TGF-β）的影响。（TGF-β）和血管内皮生长因子（VEGF）。从24名血压正常的非吸烟妇女的足月妊娠中收集人类胎盘和脐带。培养24 h后，分别加入10（-12）~ 10（-7）M的C或N，检测sFlt-1、sENG、PIGF。结果：N和C均能抑制TC释放sFlt-1、sENG和P1 GF，抑制HUVEC释放TGF-β。相反，N和C增加P1 GF分泌，而N单独增加HUVEC释放sFlt-1。结论：N和C可影响TC和HUVEC释放的一些重要血管活性因子的平衡。这可能是吸烟降低妊娠期高血压疾病风险的可能机制之一，也是众所周知的吸烟对胎儿发育的有害影响的原因之一。(C)2010爱思唯尔有限公司保留所有权利。
Objective: To examine nicotine (N) and cotinine (C) effects on trophoblast cells (TCs) and human umbilical vein endothelial cells (HUVEC) secretion of soluble fms-like tyrosine kinase (sFlt-1), soluble endoglin (sENG), placental growth factor (PIGF), transforming growth factor-beta (TGF-beta) and vascular endothelial growth factor (VEGF).Study design: Human placentas and umbilical cords were collected from uncomplicated pregnancies at term from a total of 24 non-smoking women with a history of normal blood pressure. TCs and HUVEC were cultured for 24 h with C or N (from 10(-12) to 10(-7) M).Main outcome measures: sFlt-1, sENG, PIGF. TGF-beta and VEGF release and messenger RNA (mRNA) expression were evaluated by ELISA and real-time polymerase chain reaction (PCR), respectively.Results: N and C reduced sFlt-1, sENG and P1GF release by TCs and TGF-beta release by HUVEC. Conversely, N and C increased P1GF secretion, while N alone increased sFlt-1 release by HUVEC. N and C were able to modulate VEGF mRNA expression in HUVEC.Conclusions: Our results suggest that N and C affect the balance of some important vasoactive factors released by TCs and HUVEC. This might be one of the possible mechanism through which smoke reduces the risk of hypertensive disorders during pregnancy as well as contributes to the well known detrimental effects of smoking on fetal development. (C) 2010 Elsevier Ltd. All rights reserved.