The Effect of Selenium and Polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on Immune Response in Chicken Spleen Under Heat Stress
The Effect of Selenium and Polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on Immune Response in Chicken Spleen Under Heat Stress
复制标题
DOI：
10.1007/s12011-014-0056-y
复制
发表时间：
2014-08-01
影响因子：
3.9
通讯作者：
Tian, Yunbo
中科院分区：
文献类型：
--
作者：
Xu, Danning;Li, Wanyan;Tian, Yunbo
本研究旨在探讨白术多糖硒(Se)的作用。(PAMK)，以及Se和PAMK联合应用对热应激(HS)条件下鸡脾免疫反应、热休克蛋白(HSP)水平的影响。将200只雏鸡随机分为HS组和对照组(CON)。然后分别用Se(0.3 mg/kg)、PAMK(200 mg/kg)和Se(0.3 mg/kg)+PAMK(200 mg/kg)处理。测定鸡脾组织中抗氧化酶、细胞因子含量以及HSP27和HSP70的表达水平。结果表明，HS可使小鼠脾组织中肿瘤坏死因子-α、IL-4、热休克蛋白27、热休克蛋白70和丙二醛水平升高，而干扰素-γ、IL-2、GPx和超氧化物歧化酶水平降低(P<0.05)。Se、PAMK单独及Se和PAMK合用(P<；0.05)均可改善上述反应。结果表明，在一般情况下，Se和PAMK可通过将某些细胞因子水平提高到适当水平来改善免疫反应；而在HS条件下，Se和PAMK可改变细胞因子的异常水平和氧化损伤，从而减轻HS所致的损伤。此外，Se和PAMK对鸡脾中这些生物标志物的调控存在协同作用。因此，Se和PAMK在鸡的免疫功能调节中起着重要的作用。考虑到PAMK对免疫调节的协同作用，该草药在改善传统免疫调节剂的作用方面值得进一步研究。
The aim of the present study was to investigate the effect of selenium (Se), polysaccharide of Atractylodes macrocephala Koidz. (PAMK), and the combination of Se and PAMK on the immune response, heat shock protein (HSP) levels under heat stress (HS) condition in chicken spleen. Two hundred chickens were randomly divided into two groups, the HS group and the control (Con) group. Then these chickens were treated with Se (0.3 mg/kg), PAMK (200 mg/kg) alone, and the combination of Se (0.3 mg/kg) and PAMK (200 mg/kg). The antioxidative enzymes, cytokines contents, and expression levels of HSP27 and HSP70 were examined in chicken spleen. The results indicated that HS induced higher levels of TNF-alpha, IL-4, HSP27, HSP70, and MDA levels but lower level of IFN-gamma, IL-2, Gpx, and SOD in spleen (P < 0.05). These responses were ameliorated by the treatment of Se, PAMK alone, and the combination of Se and PAMK (P < 0.05 or not) The results showed that under common condition, Se and PAMK could improve the immune response by enhancing the levels of some cytokines to proper levels; however, under HS condition, Se and PAMK could change the abnormal levels of cytokines and oxidative damages to ameliorate the injury induced by HS. In addition, there existed synergistic effect on the modulation of these biomarkers in chicken spleen between Se and PAMK. So both Se and PAMK play important roles in regulating the immune function in chicken. Considering the synergistic effect on immune regulation of PAMK, this herb deserves further investigation to evaluate its role in improving the effect of traditional immune regulators.