Development and Use of Fluorescent Nanodiamonds as Cellular Markers
Development and Use of Fluorescent Nanodiamonds as Cellular Markers
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DOI：
10.1007/978-1-4419-0531-4_6
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Chang, Huan-Cheng
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文献类型：
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作者：
Chang, Huan-Cheng
金刚石是碳的同素异形体。区别于其他碳材料的一个独特特性是金刚石是光学透明的，并且通常含有作为色心的点缺陷。氮空位（N-V）缺陷是金刚石中最值得注意的色心。这些中心可以通过离子束照射，然后通过热退火可重复地产生，并且当被可见光激发时可以发射强且稳定的荧光。这种独特的光学性质与材料的非细胞毒性和良好的表面功能化特性相结合，使纳米金刚石成为细胞环境中生物成像应用的有前途的荧光探针。本文总结了我们在生产和表征明亮的多色（红色和绿色）荧光纳米金刚石（FND）及其作为细胞标记物的应用方面所做的努力。这条生产线上技术的显著进步沿着包括FND的大规模生产和活细胞中单个35 nm红色FND颗粒的三维真实的实时跟踪。我们设想，该材料的进一步开发将为FND在生物学和医学中的富有成效的应用提供更高的灵敏度和更好的能力。
Diamond is an allotrope of carbon. A unique property that distinguishes it from the other carbon materials is that diamond is optically transparent and often contains point defects as color centers. Nitrogen vacancy (N-V) defects are the most noteworthy color centers in diamond. These centers can be produced reproducibly by ion beam irradiation, followed by thermal annealing, and can emit strong and stable fluorescence when excited by visible light. This unique optical property combined with the non-cytotoxicity and good surface functionalizability characteristics of the material makes nanoscale diamonds a promising fluorescent probe for bioimaging applications in cellular environments. This article summarizes the results of our efforts in production and characterization of bright, multicolored (red and green) fluorescent nanodiamonds (FNDs) and their use as cellular markers. Notable advancement of technologies along this line includes mass production of FNDs and real time tracking of a single 35-nm red FND particle in three dimensions in live cells. We envision that further development of the material will provide an increased sensitivity and improved capability for fruitful applications of FNDs in biology and medicine.