ELECTRON MICROSCOPY OF PLASMOLYSIS IN ESCHERICHIA COLI

ELECTRON MICROSCOPY OF PLASMOLYSIS IN ESCHERICHIA COLI
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DOI:
10.1128/jb.85.3.499-503.1963
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发表时间:
1963-01-01
影响因子:
3.2
通讯作者:
COTAROBLES, EH
COTAROBLES, EH
中科院分区:
生物学3区
文献类型:
--
作者:
COTAROBLES, EH

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在0.35[图像]蔗糖中进行质解的大肠杆菌显示在细胞的一端或在原生质体分裂完成的分裂细胞的中心进行质解。中央质裂表明原生质体分离可以在细胞壁内陷完成之前完成。这些研究支持了细胞收缩分裂的观点。细胞壁和细胞质之间的结合强度在整个细胞内是不均匀的。它在这些杆状细胞的两侧最强,在单个细胞的一端最弱。因此,单个细胞通常形成一个杯状液泡,其中细胞质从细胞的一端坍塌。
Escherichia coli plasmolyzed in 0.35 [image] sucrose reveal plasmolysis at one tip of a cell or in the center of dividing cells in which protoplast partition has been complete. Central plasmolysis reveals that protoplast separation can be completed before the invagination of the cell wall is complete. These studies support the concept that these cells divide by constriction. The strength of the union between cell wall and cytoplasm is not uniform around the entire cell. It is strongest along the sides of these rod-shaped cells and weakest at one tip of the single cell. Thus, a single cell generally forms one cup-shaped vacuole in which the cytoplasm has collapsed away from one tip of the cell.