S-S bonds are not required for the sonochemecial formation proteinaceous microspheres: the case of streptavidin

S-S bonds are not required for the sonochemecial formation proteinaceous microspheres: the case of streptavidin
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DOI:
10.1042/bj20020676
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发表时间:
2002-09-15
影响因子:
4.1
通讯作者:
Gedanken, A
Gedanken, A
中科院分区:
生物学3区
文献类型:
--
作者:
Avivi, S;Gedanken, A

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采用声化学方法可以制备蛋白质微球。然而,众所周知,这些蛋白质必须至少含有一个半胱氨酸残基,才能使用这种方法获得稳定的微球。在本研究中,我们以不含半胱氨酸残基的链霉亲和素为原料,采用声化学方法制备了链霉亲和素微球。
Proteinaccous microspheres can be prepared using the sono-chemical method. However, it is known that these proteins should possess at least one cysteine residue in order to obtain stable microspheres using this method. In the present study, we have produced streptavidin microspheres, using the sono-chemical method, from streptavidin, which does not have any cysteine residues.