A haemophilus-like organism isolated from pig lung and the associated pneumonic lesions.
A haemophilus-like organism isolated from pig lung and the associated pneumonic lesions.
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DOI：
10.1016/s0368-1742(57)80031-9
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发表时间：
1957-10-01
影响因子：
0.8
通讯作者：
ELLIOT, J
中科院分区：
文献类型：
--
作者：
PATTISON, I H;HOWELL, D G;ELLIOT, J
A类嗜血杆菌，与H.猪流感病毒与猪瘟病毒一起从患肺炎的猪肺中分离出来。这是一个小的，革兰氏阴性，多形性杆显示丝状形式和包囊的证据。它是非能动的，过氧化氢酶阴性。它的殖民地形式类似于H。与金黄色葡萄球菌一起生长时，它也表现出随体现象。然而，它并不像H那样需要因子×和V。infuenzae，但确实需要在血液、血清和商业酵母提取物中发现的因子，在7头猪中的任何一头猪中，对微生物培养物的肠内注射均未引起疾病，并且在接种后14至25天处死这些动物进行检查时，未从这些动物中分离出微生物，对来自原始肺的未过滤物质的肠内注射，或与猪瘟病毒一起的微生物培养物，在10头猪中的7头中引起肺炎和胸膜炎;除3头未显示胸膜炎外，从所有这些肺中回收了微生物。单纯经气管内注射猪瘟病毒引起的肺炎在显微镜下表现为塌陷，伴有不同程度的细胞渗出物。由猪瘟病毒（一种嗜血杆菌样生物体）经气管内注射引起的肺炎，在显微镜下的特征是与猪瘟病毒有关的变化加上一个结节性病变，详细描述了结节性病变，并与嗜血杆菌有关，以及广泛的胸膜炎。
AHaemophilus likeorganism, not apparently identical withH. influenzaesuis, was isolated together with swine fever virus from a pneumonic pig lung. It was a small, Gram-negative, pleomorphic rod showing filamentous forms and evidence of capsulation. It was non-motile and catalase negative. Its colonial form was similar toH. infiuenzaeand, like the latter, it showed satellitism when growing in association withStaphylococcus aureus. It did not, however, require factors × and V as described forH. infiuenzae, but did require a factor to be found in blood, serum, and commercial yeast extract, Intratracheal injection of cultures of the organism failed to cause disease in any of 7 pigs and the organism was not isolated from these animals when they were killed for examination 14 to 25 days after inoculation, întratracheal injection of unfiltered material from the original lung, or of cultures of the organism together with swine fever virus, caused pneumonia and pleurisy in 7 of 10 pigs; the organism was recovered from all these lungs except the 3 not showing pleurisy. The pneumonia caused by intratracheal injection of swine fever virus alone was characterized microscopically by collapse, with a varying degree of cellular exudate. The pneumonia caused by intratracheal injection of swine fever virus olus the Haemophilus likeorganism was characterized microscopically by the changes associated with swine fever virus plus a nodular lesion which is described in detail and was associated with theHaemophilus, and by extensive pleurisy.