RNA-seq analysis reveals the significant effects of different light conditions on oil degradation by marine Chlorella vulgaris
RNA-seq analysis reveals the significant effects of different light conditions on oil degradation by marine Chlorella vulgaris
复制标题
RNA-seq分析揭示了不同光照条件对海洋小球藻降解油的显着影响
DOI：
10.1016/j.marpolbul.2018.10.021
复制
发表时间：
2018-12-01
影响因子：
5.8
通讯作者：
Mu, Jun
中科院分区：
文献类型：
--
作者：
Li, Jingjing;Chen, Qingguo;Mu, Jun
海洋小球藻是一种高效的碳氢化合物降解菌，易受光照影响。本研究考察了不同光照条件对普通拟青霉及其粗酶降解原油的直接影响。在光照12h的条件下，粗酶的降解率提高了39.36%，而添加该酶和普通假丝酵母的降解率提高了121.73%。反之，异养条件下加酶与普通乳杆菌对石油的降解率呈负相关，降解率由74.32%降至48.65%，降解率进一步降低34.54%。RNA测序分析结果表明，普通弧菌在异养生理状态下，碳氢化合物的去除主要归因于代谢作用。而在混合营养生理状态下，由于普通弧菌代谢和光催化氧化的耦合，提高了碳氢化合物的去除效率。功能酶在光催化和生物降解过程中起着关键作用。
Marine Chlorella vulgaris, an efficient hydrocarbon-degrading organism, is easily affected by light. In this study, we investigated the direct effects of different light conditions on crude oil degradation by C. vulgaris and its crude enzyme. Under 12 h illumination, the crude enzyme improved hydrocarbon removal by 39.36%, whereas the addition of the enzyme and C. vulgaris increased the degradation rate by 121.73%. Conversely, the addition of enzyme under heterotrophic condition was negatively related to oil degradation by C. vulgaris, and the degradation rate decreased from 74.32% to 48.65% and further reduced by 34.54%. The results of RNA sequencing analysis suggested that hydrocarbons removal was attributed to C. vulgaris metabolism in heterotrophic physiological state. While enhanced removal efficiency of hydrocarbons was achieved in mixotrophic physiological state due to the coupling of C. vulgaris metabolism with photocatalytic oxidation. Functional enzymes played key roles in photocatalysis and biodegradation processes.