Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines.
Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines.
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DOI：
10.1038/bjc.1994.161
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发表时间：
1994-05
影响因子：
8.8
通讯作者：
Carter DC
中科院分区：
文献类型：
--
作者：
Falconer JS;Ross JA;Fearon KC;Hawkins RA;O'Riordain MG;Carter DC
许多多不饱和脂肪酸已被证明可以在体外抑制恶性细胞的生长。为了研究脂肪酸是否会改变人胰腺癌的生长，将月桂酸、硬脂酸、棕榈酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、花生四烯酸、二十二碳六烯酸和二十碳五烯酸 (EPA) 酸分别与 MIA PaCa-2、PANC-1 和 CFPAC 细胞系一起孵育，浓度范围为 1.25 µM 至 50 µM，以及每种脂肪酸对细胞的影响检查生长情况。所有测试的多不饱和脂肪酸均具有抑制作用，其中 EPA 最为有效（ID50 2.5-5 microM）。单不饱和或饱和脂肪酸没有抑制作用。 EPA 的作用可以通过抗氧化维生素 E 醋酸酯或油酸来逆转。环加氧酶抑制剂吲哚美辛和吡罗昔康对EPA的作用没有影响。 EPA 的作用似乎与脂质过氧化物的产生有关，尽管脂质过氧化水平并不总是与细胞死亡的程度直接相关。某些脂肪酸在体外以体内可达到的浓度显着抑制三种人胰腺癌细胞系生长的能力表明，施用此类脂肪酸可能对胰腺癌患者具有治疗益处。
A number of polyunsaturated fatty acids have been shown to inhibit the growth of malignant cells in vitro. To investigate whether fatty acids modify the growth of human pancreatic cancer, lauric, stearic, palmitic, oleic, linoleic, alpha-linolenic, gamma-linolenic, arachidonic, docosahexaenoic and eicosapentaenoic (EPA) acids were each incubated with the cells lines MIA PaCa-2, PANC-1 and CFPAC at concentrations ranging from 1.25 microM to 50 microM and the effect of each fatty acid on cell growth was examined. All the polyunsaturated fatty acids tested had an inhibitory effect, with EPA being the most potent (ID50 2.5-5 microM). Monounsaturated or saturated fatty acids were not inhibitory. The action of EPA could be reversed with the anti-oxidant vitamin E acetate or with oleic acid. The cyclo-oxygenase inhibitors indomethacin and piroxicam had no effect on the action of EPA. The action of EPA appeared to be associated with the generation of lipid peroxides, although the level of lipid peroxidation did not always appear to correlate directly with the extent of cell death. The ability of certain fatty acids to inhibit significantly the growth of three human pancreatic cancer cell lines in vitro at concentrations which could be achieved in vivo suggests that administration of such fatty acids may be of therapeutic benefit in patients with pancreatic cancer.