An atlas of glycine- and GABA-like immunoreactivity and colocalization in the cochlear nuclear complex of the guinea pig
An atlas of glycine- and GABA-like immunoreactivity and colocalization in the cochlear nuclear complex of the guinea pig
复制标题
豚鼠耳蜗核复合体中甘氨酸和 GABA 样免疫反应性和共定位图谱
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Jon Storm
中科院分区：
文献类型：
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作者：
J. Kolston;K. Osen;Carole M. Hackney;O. Ottersen;Jon Storm
本文研究了γ-氨基丁酸（GABA）和甘氨酸样免疫反应物在豚鼠耳蜗核复合体中的分布和共定位。所使用的方法是基于包埋后免疫细胞化学的0.5 μ m厚的塑料切片对处理的多克隆抗体结合的GABA和甘氨酸分别。免疫反应细胞，推测短轴突神经元，主要在耳蜗背核，主要是单GABA标记的细胞在浅层，双标记的中间，和单甘氨酸标记的深层。少数大的单甘氨酸标记的细胞，被解释为连合神经元，发生在腹侧核。散在的双标记细胞，可能是高尔基细胞，被认为是在颗粒细胞域。所有三种染色类别的免疫标记斑点在整个复合体中大量发生，与胞体和神经元并列，显示出不同类型神经元的差异分布。三个免疫标记的道：tuberculoventral道，连合声纹，梯形下降纤维。大多数的纤维在这些道单标记的甘氨酸，虽然在最后提到的道单-GABA-和双标记的纤维也发现。这里描述的一些免疫标记的细胞类型提出了类似的标记点和纤维的起源的基础上已知的内在连接。
SummaryThe distribution and colocalization of γ-aminobutyric acid (GABA)- and glycine-like immunoreactivity in the cochlear nuclear complex of the guinea pig have been studied to produce a light microscopic atlas. The method used was based on post-embedding immunocytochemistry in pairs of 0.5-μm-thick plastic sections treated with polyclonal antibodies against conjugated GABA and glycine respectively. Immunoreactive cells, presumably short axon neurones, predominated in the dorsal cochlear nucleus, with mostly single-GABA-labelled cells in the superficial layer, double-labelled in the middle, and single-glycine-labelled in the deep layers. A few large single-glycine-labelled cells, interpreted as commissural neurons, occurred in the ventral nucleus. Scattered double-labelled cells, probably Golgi cells, were seen in the granule cell domain. Immunolabelled puncta of all three staining categories occurred in large numbers throughout the complex, apposed to somata and in the neuropil, showing a differential distribution onto different types of neuron. Three immunolabelled tracts were noted: the tuberculoventral tract, the commissural acoustic stria, and the trapezoidal descending fibres. Most of the fibres in these tracts were single-labelled for glycine, although in the last mentioned tract single-GABA- and double-labelled fibres were also found. Some of the immunolabelled cell types described here are proposed as the origins of the similarly labelled puncta and fibres on the basis of known intrinsic connections.
豚鼠从耳蜗核到上橄榄的摄取和逆行转运[3H]GABA。
影响因子：
2.8
作者：
Ostapoff,EM;Morest,DK;Potashner,SJ
通讯作者：
Potashner,SJ