Apatite-forming ability (bioactivity) of ProRoot MTA
Apatite-forming ability (bioactivity) of ProRoot MTA
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DOI：
10.1111/j.1365-2591.2010.01768.x
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发表时间：
2010-10-01
影响因子：
5
通讯作者：
Prati, C.
中科院分区：
文献类型：
--
作者：
Gandolfi, M. G.;Taddei, P.;Prati, C.
作为生物活性（与骨结合能力）的指标，我们在prooroot MTA水泥中浸泡于含磷酸盐溶液（DPBS）后测试了P> aimapatite形成能力。方法制备磁盘样品，分别浸泡于DPBS中10 min、5 h、1和7 d。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、微拉曼光谱（micro-Raman spectroscopy）和环境扫描电镜（ESEM-EDX）对水泥表面进行了研究。同时还考察了贮存液的pH值。结果DPBS浸泡5 h后，光谱学分析显示磷酸钙条带。1 d后，通过ESEM/EDX观察到由直径0.1 ~ 0.8微米的磷灰石球晶组成的均匀涂层。7 d后，其厚度有所增加。浸渍5 h后磷灰石已成核。此时，还观察到水泥表面存在波特兰铁矿(即Ca（OH)(2)，氢氧化钙）；在较长的时间内，该成分被释放到培养基中，培养基的pH值显著升高。结论：该研究证实了prooroot MTA在数小时内形成磷灰石表层的能力。prooroot MTA优异的生物活性可能比传统的根端或根穿孔修复材料具有显著的临床优势。
P>AimApatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS).MethodologyDisk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, by micro-Raman spectroscopy and by environmental scanning electron microscope with energy dispersive X-ray (ESEM-EDX) analyses. The pH of the storage solution was also investigated.ResultsSpectroscopic analyses revealed calcium phosphate bands after 5-h immersion in DPBS. After 1 day, an even coating composed of apatite spherulites (0.1-0.8 micron diameter) was observed by ESEM/EDX. After 7 days, its thickness had increased. Apatite nucleation had already occurred after 5-h immersion. At this time, the presence of portlandite (i.e. Ca(OH)(2), calcium hydroxide) on the cement surface was also observed; at longer times, this component was released into the medium, which underwent a remarkable pH increase.ConclusionsThe study confirms the ability of ProRoot MTA to form a superficial layer of apatite within hours. The excellent bioactivity of ProRoot MTA might provide a significant clinical advantage over the traditional cements used for root-end or root-perforation repair.