LongSAGE analysis revealed the presence of a large number of novel antisense genes in the mouse genome
LongSAGE analysis revealed the presence of a large number of novel antisense genes in the mouse genome
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DOI：
10.1093/bioinformatics/bti205
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发表时间：
2005-04-15
期刊：
影响因子：
5.8
通讯作者：
Imai, K
中科院分区：
文献类型：
--
作者：
Wahl, MB;Heinzmann, U;Imai, K
动机：尽管越来越多的观念的功能重要性反义转录在基因调控，全基因组范围内的概述反义基因的本体论尚未获得。因此，我们试图找到新的反义基因，通过使用我们的LongSAGE数据集的202 015标签（包括41 718个独特的tags），实验产生的小鼠胚胎tail libraries. Results：我们确定了1260个潜在的反义基因，其中1001没有在EnsEMBL注释，从而被认为是新的。有趣的是，他们的正义同行共表达在大多数cases.Conclusions：使用LongSAGE转录组数据是非常强大的，在识别的thus-迄今未知的反义转录，即使在良好的特点的生物体，如小鼠的情况下。
Motivation: Despite the increasing notions of the functional importance of antisense transcripts in gene regulation, the genome-wide overview on the ontology of antisense genes has not been obtained. Therefore, we tried to find novel antisense genes genome-wide by using our LongSAGE dataset of 202 015 tags (consisting of 41 718 unique tags), experimentally generated from mouse embryonic tail libraries.Results: We identified 1260 potential antisense genes, of which 1001 are not annotated in EnsEMBL, thereby being regarded as novel. Interestingly their sense counterparts were co-expressed in the majority of the cases.Conclusions: The use of LongSAGE transcriptome data is extremely powerful in the identification of thus-far unknown antisense transcripts, even in the case of well-characterized organisms like the mouse.